Sigma-Aldrich Silica Gel 60 F254 100 Hpt

Material

glass support
silica gel 60 matrix

Quality Level

100

Feature

binder Organic Polymer
fluorescent indicator

Packaging

pkg of 25 plates

Technique(S)

thin layer chromatography (TLC): suitable

Layer Thickness

200 μm

Plate L × W

10 cm × 10 cm

Particle Size

5-6 μm

Pore Size

60 Å medium pore diameter

Storage Temp.

2-30°C

Related Categories

Thin Layer Chromatography (TLC)

General Description

These pre-scored glass plates fold into smaller formats with just one easy snap of the fingers.ex Glass plates 10 x 10 cm

Linkage

Replaces: 5635-5; 5635

Analysis Note

Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 5 - 7 µm
Layer thickness: 150 - 200 µm
Deviation of layer thickness per plate: ≤ 35 µm
Chromatographic testings:
A) Colour test
hRf-values
- bleu vif organol, colour test, lipophile:
17 - 22
- Ceres Black G, colour test, lipophile: 38 - 43
- ceres Violet brn, colour test, lipophile: 58 - 64
separation number
- colourtest, lipophile:
≥ 10.0
B) Steriod test
hRf-values
- hydrocortisone, steroid test:
23 - 29
- Reichstein S, steroid test: 36 - 45
- methyltestosterone, steroid test: 43 - 52
separation number
- steroid test:
≥ 10.5
Typical value determined on a conditioned plate
Eluent A) Toluene (45% rel.humidity)
Eluent B) Ethyl acetate/Toluene (95+5 v/v; 20% rel.humidity)