Sigma-Aldrich Silica Gel 60 F254 25 Tlc

Material

glass support
silica gel 60 matrix

Quality Level

100

Feature

binder Organic Polymer
fluorescent indicator

Packaging

pkg of 25 plates

Technique(S)

thin layer chromatography (TLC): suitable

Layer Thickness

250 μm

Plate L × W

5 cm × 20 cm

Particle Size

10-12 μm

Pore Size

60 Å medium pore diameter

Storage Temp.

2-30°C

Related Categories

Thin Layer Chromatography (TLC)

General Description

25 Glass plates 5 x 20 cmOur classical silica TLC plates are based on a combination of our proven silica gel 60 and the addition of a unique polymeric binder resulting in a very adherent and hard surface that will not crack or blister and even allow writing with a pensil on the surface without risk to damage the layer.

Application

Glass TLC plate, silica gel coated with flourescent indicator F254. The silica gel TLC plates come in a quantity of 25 size 5x20 cm for thin layer chromatography applications.

Linkage

Replaces: 5808-3; 5808

Analysis Note

Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 9.7 - 11.7 µm
Layer thickness: 210 - 270 µm
Deviation of layer thickness per plate: ≤ 35 µm
Chromatographic testings:
A) Colour test
hRf-values
- bleu vif organol, colour test, lipophile:
11 - 25
- Ceres Black G, colour test, lipophile: 34 - 48
- ceres Violet brn, colour test, lipophile: 52 - 67
separation number (colourtest, lipophile): ≥ 10.5
B) Steriod test
hRf-values
- hydrocortisone, steroid test:
25 - 37
- Reichstein S, steroid test: 37 - 49
- methyltestosterone, steroid test: 42 - 54
Typical value determined on a conditioned plate
Eluent A) Toluene (45% rel.humidity)
Eluent B) Ethyl acetate/Toluene (95+5 v/v; 20% rel.humidity)