Cell Signaling Erf1 Antibody

Name: Cell Signaling Erf1 Antibody
SKU: CSIG-13916S
Price: 309.06 USD

List Price $309.06 Your Price $309.06

Cell Signaling Erf1 Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-13916S

MFG.PART: 13916S

UNSPSC: 41105803

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	50
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	eRF1 Antibody recognizes endogenous levels of total eRF1 protein.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe117 of human eRF1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background	Efficient termination of mRNA translation in eukaryotes is dependent upon a complex of two polypeptide release factors, eRF1 and eRF3 (1). The eukaryotic translation termination factor 1 (eRF1, ETF1) structurally resembles tRNA, which allows it to participate in stop codon recognition as well as hydrolysis of the peptidyl-tRNA conjugate (2,3). The eRF1 protein contains three functionally distinct domains, including an amino-terminal domain that harbors discrete motifs that participate in stop codon recognition (4,5). Lysine hydroxylation within the amino-terminal domain is required for efficient termination of mRNA translation (6). The central region of eRF1 harbors a GGQ motif that facilitates hydrolysis of peptidyl-tRNA conjugates (7), while its carboxy terminus participates in eRF3 binding (8).

Specifications