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Cell Signaling Phospho-Alk (Tyr1507) (D6f1v) Rabbit mAb
List Price
$405.01
Your Price
$405.01
Cell Signaling Phospho-Alk (Tyr1507) (D6f1v) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-14678S
MFG.PART:
14678S
UNSPSC:
41116133
Manufacturer:
Cell Signaling
| Size | 100 µl |
| Reactivity | H |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 80 (NPM-ALK), 220 (ALK) |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:100, Immunofluorescence (Immunocytochemistry): 1:400, Flow Cytometry (Fixed/Permeabilized): 1:200} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Phospho-ALK (Tyr1507) (D6F1V) Rabbit mAb recognizes endogenous levels of ALK protein only when phosphorylated at Tyr1507 (equivalent to Tyr567 of NPM-ALK). |
| Species Reactivity | Human |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1507 of human ALK protein. |
| Background | Anaplastic lymphoma kinase (ALK) is a tyrosine kinase receptor for pleiotrophin (PTN), a growth factor involved in embryonic brain development (1-3). In ALK-expressing cells, PTN induces phosphorylation of both ALK and the downstream effectors IRS-1, Shc, PLCγ, and PI3 kinase (1). ALK was originally discovered as a nucleophosmin (NPM)-ALK fusion protein produced by a translocation (4). Investigators have found that the NPM-ALK fusion protein is a constitutively active, oncogenic tyrosine kinase associated with anaplastic lymphoma (4). Research literature suggests that activation of PLCγ by NPM-ALK may be a crucial step for its mitogenic activity and involved in the pathogenesis of anaplastic lymphomas (5).A distinct ALK oncogenic fusion protein involving ALK and echinoderm microtubule-associated protein like 4 (EML4) has been described in the research literature from a non-small cell lung cancer (NSCLC) cell line, with corresponding fusion transcripts present in some cases of lung adenocarcinoma. The short, amino-terminal region of the microtubule-associated protein EML4 is fused to the kinase domain of ALK (6-8).Phosphorylation of ALK on Tyr1507 was identified at Cell Signaling Technology using PhosphoScan®, an LC-MS/MS platform used for phosphorylation site discovery (6). Phosphorylation of ALK at Tyr1507 (Tyr567 in NPM-ALK) has been shown to be important for interaction with the adaptor proteins Shc, FRS2-α, and FRS2-β (9,10). |
| SKU | CSIG-14678S |
|---|---|
| Supplier Part Number | 14678S |
| UM | EA |
| UNSPSC | 41116133 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |