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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R, H | WB, IP | Rb | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-784R |
| Description | Anti-IRS1 Antibody, clone 58-10C-31, rabbit monoclonal |
| Alternate Names |
|
| Background Information | IRS1 (Insulin Receptor Substrate 1) transmits insulin signals via metabolic and mitogenic pathways. It is heavily phosphorylated on both serine and tyrosine residues. These phosphorylated tyrosines enable IRS to act as a docking protein that binds SH2 domains of such proteins as PI3 Kinase (phosphatidylinositol 3-kinase) and GRB2, resulting in activation. Over expression and phosphorylation of serine is associated with insulin resistance and breast cancer. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified Rabbit Monoclonal IgG Supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide and 40% glycerol. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect IRS1 using this Anti-IRS1 Antibody, clone 58-10C-31 validated for use in WB & IP. |
| Key Applications |
|
| Application Notes | Western Blot (SNAP ID) Analysis: 0.1 µg/mL from a previous lot detected IRS-1 on 10 µg of 3T3/A31, 3T3/L1, L6 and MCF7 cell lysates. Immunoprecipitation Analysis: 1 µg from a previous lot immunoprecipitated IRS-1 from 100 µg of MCF7 cell lysate. Arrow indicates IRS-1 (~160 kDa). There is cross reactivity of the detection antibody with the heavy chaing of Rabbit IgG as shown at ~50 kDa. |
| Biological Information | |
|---|---|
| Immunogen | KLH- conjugated - linear peptide corresponding to the C-terminus of rat IRS1. |
| Epitope | C-terminus |
| Clone | 58-10C-31 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Antibody recognizes the C-terminus of IRS1. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Reacts with mouse, rat and human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit. Ref.3 SUBUNIT STRUCTURE: Interacts with SOCS7 By similarity. Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR via the PTB domain. Binds to phosphatidylinositol 3-kinase p85 subunit via the phosphorylated YXXM motifs. Binds ROCK1. PTM: Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-307 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor, and Ser-789 phosphorylation is increased in the liver of insulin-resistant rats. Phosphorylation of Tyr-895 is required for GRB2-binding. SEQUENCE SIMILARITIES: Contains 1 IRS-type PTB domain. Contains 1 PH domain. |
| Molecular Weight | ~ 160 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in 3T3/A31, 3T3/L1, L6 or MCF7 cell lysates. Western Blot Analysis: 0.1 µg/mL of this antibody detected IRS-1 on 10 µg of 3T3/A31, 3T3/L1, L6, or MCF7 cell lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |