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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | ELISA, NEUT, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB2148P |
| Replaces | AB1450P |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Tumor Necrosis Factor-α Antibody |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Immunoglobulin purified by affinity chromatography employing immobilized mTNF-alpha matrix. A sterile filtered antibody solution (1mg/mL) was lyophilized from 0.5X PBS, pH 7.2. Reconstitute with sterile distilled water. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Tumor Necrosis Factor-α using this Anti-Tumor Necrosis Factor-α Antibody validated for use in ELISA, NEUT & WB. |
| Key Applications |
|
| Application Notes | ELISA: A concentration of at least 0.5μg/mL (100μL/well) of AB2148P is required to detect 0.2-0.4 ng/well of recombinant mTNF-alpha. Western Blot: A concentration of 0.1-0.2 μg/mL of AB2148P is required to detect 1.5-3.0 ng/lane of mTNF-alpha, under either reducing or non-reducing conditions. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mTNF-a (0.50 ng/ml), a concentration of 0.010-0.015 μg/ml of AB2148P is required. Optimal working dilutions must be determined by the end user |
| Biological Information | |
|---|---|
| Immunogen | Highly pure (>98%) recombinant Mouse TNF-alpha. |
| Host | Rabbit |
| Specificity | Reacts with Mouse Tumor Necrosis Factor-alpha (TNF-alpha), a potent immuno-modulator that mediates a wide range of immune and inflammatory responses including the activation of B and T cells. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P01375 # Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. SIZE: 233 amino acids; 25644 Da SUBUNIT: Homotrimer. SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein. & Tumor necrosis factor, soluble form: Secreted. PTM: The soluble form derives from the membrane form by proteolytic processing. & The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. DISEASE: SwissProt: P01375 # Cachexia accompanies a variety of diseases, including cancer and infection, and is characterized by general ill health and malnutrition. & Genetic variations in TNF are associated with susceptibility to psoriatic arthritis [MIM:607507]. Psoriasis is a chronic inflammatory dermatosis that affects approximately 2% of the population. It is characterized by red, scaly skin lesions that are usually found on the scalp, elbows, and knees, and may be associated with severe arthritis. Psoriatic arthritis has been defined as an inflammatory arthritis usually without any rheumatoid factor in serum (seronegative arthritis) associated with psoriasis. & Genetic variations in TNF are associated with susceptibility to hepatitis B virus infection (HBV infection) [MIM:610424]. Approximately one third of all cases of cirrhosis and half of all cases of hepatocellular carcinoma can be attributed to chronic HBV infection. HBV infection may result in subclinical or asymptomatic infection, acute self-limited hepatitis, or fulminant hepatitis requiring liver transplantation. SIMILARITY: SwissProt: P01375 ## Belongs to the tumor necrosis factor family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile water to a concentration of = 0.2 mg/mL the antibody is stable for at least six weeks at 2-4ºC. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |