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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, B | ICC, IP, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1598 |
| Replaces | 04-1066 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Post Synaptic Density Protein 95 Antibody, clone 7E3-1B8 |
| Alternate Names |
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| Background Information | The postsynaptic density (PSD) is an electron dense structure just beneath the postsynaptic membrane. Several functions have been proposed for the PSD including regulating receptor number and clustering, anchoring signal transduction molecules at the synapse and mediating adhesion between the presynaptic and postsynaptic membranes. The most abundant PSD protein is the [alpha] subunit of the type II calcium/calmodulin dependent protein kinase ([alpha]CaMKII). This protein is likely to play a role in the calcium-mediated signal transduction at the synapse that mediates certain forms of synaptic plasticity. Another major PSD protein is PSD-95, a member of the guanylate kinase family (GUK) of proteins. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Purified moust monoclonal IgG2a liquid in buffer containing PBS, pH 7.2, with 0.09% sodium azide and 50% glycerol. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Post Synaptic Density Protein 95 Antibody, clone 7E3-1B8 is an antibody against Post Synaptic Density Protein 95 for use in IC, IP & WB. |
| Key Applications |
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| Application Notes | Immunocytochemistry: A previous lot of this antibody was used in IC. Immunoprecipitation: A previous lot of this antibody was used in IP. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant rat PSD-95 |
| Clone | 7E3-1B8 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Reacts with recombinant rat PSD-95 and identifies a band at approximately ~100 kDa, corresponding to PSD-95, on western blots of rat brain, mouse brain and bovine brain extracts. Also present in rat brain and mouse brain tissue extracts are bands of approximately 80 kDa and 50 kDa. PSD-95 is believed to initiate NMDA receptor clustering on the post synaptic membrane. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Species Reactivity Note | Expected to react with human based on sequence homology |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Summary | This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. It heteromultimerizes with DLG2. With DLG2 it is recruited into the same NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. |
| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P78352 # Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ACCN3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B (By similarity). SIZE: 724 amino acids; 80495 Da SUBUNIT: Interacts through its first two PDZ domains with GRIN2A, GRIN2B, GRIN2C, GRIN2D, ACCN3, certain splice forms of GRIN1, KCND2, CXADR and SYNGAP1. Interacts through its second PDZ domain with the PDZ domain of NOS1 or the C-terminus of CAPON. May interact with HTR2A. Interacts through its guanylate kinase-like domain with DLGAP1/GKAP, DLGAP2, DLGAP3, DLGAP4, MAP1A, and BEGAIN (By similarity). Interacts through its first two PDZ domains with KCNA1, KCNA2, KCNA3, KCNA4 and ERBB4. Interacts through its first PDZ domain with GRIK2, KCNA4 and CRIPT. Interacts through its third PDZ domain with NLGN1, and probably with NLGN2 and NLGN3. Interacts through its guanylate kinase-like domain with KIF13B. Isoform 2 interacts through an L27 domain with HGS/HRS and the first L27 domain of CASK. SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Note=High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells. TISSUE SPECIFICITY: Brain. DOMAIN: SwissProt: P78352 The PDZ domain 3 mediates interaction with ADR1B. & The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to post-synaptic density. PTM: Palmitoylation of isoform 1 is required for targeting to postsynaptic density (By similarity). SIMILARITY: Belongs to the MAGUK family. & Contains 1 guanylate kinase-like domain. & Contains 3 PDZ (DHR) domains. & Contains 1 SH3 domain. |
| Molecular Weight | ~ 95 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot on Mouse brain lysates. Western Blot Analysis: 1:500 dilution of this antibody detected PSD-95 on 10 µg of Mouse brain lysates. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20ºC from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |