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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ch, Gp, H, M, R, Rb, Po, Ma | IHC, IP, WB, ChIP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1622 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Spectrin alpha chain (nonerythroid) Antibody, clone AA6 |
| Alternate Names |
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| Background Information | Non-erythroid Spectrin, also known as Fodrin, plays a role in stabilizing membrane structures, maintaining cell shape and linking the cytoskeleton to plasma membranes or intracellular vesicles. As one of the primary targets cleaved by activated caspases during apoptosis, the full-length ~240 kDa protein can be cleaved to yield an N-terminal product of ~150 kDa, and C-terminal products of ~120 and ~35 kDa. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1 in buffer containing PBS with 1% BSA and 0.09% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Spectrin alpha chain (nonerythroid) Antibody, clone AA6 is an antibody against Spectrin alpha chain (nonerythroid) for use in IH, IP, WB, ChIP. |
| Key Applications |
|
| Applications Not Recommended |
|
| Application Notes | Immunohistochemistry (IFA): A 1:200-1:400 dilution of a previous lot was used in immunohistochemistry. Does not work on paraffin sections. Optimal working dilutions must be determined by end user. Chromatin Immunopreciptiation: A previous lot of this antibody was used to immunoprecipitate Spectrin Alpha from chromatin-associated protein extracts derived from normal human, HeLa, FA-A, and transduced FA-A cells (McMahon, L.W., et al. (1999). J. Biological Chem. 274:32904-32908). |
| Biological Information | |
|---|---|
| Immunogen | Chicken red blood cell membranes purified by hypotonic lysis and mechanical enucleation. |
| Clone | AA6 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Specific for the 240, 150, and 120 kDa forms of alpha-spectrin/alpha-fodrin molecule of all mammalian nonerythroid cells and chicken alpha-spectrin. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Species Reactivity Note | Chicken, Guinea Pig, Human, Mouse,Pig, Rat, and Rabbit. Wide-mammalian reactivity is expected. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q13813 # Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane. SIZE:2472 amino acids; 284539 Da SUBUNIT: Like erythrocyte spectrin, the spectrin-like proteins are capable of forming dimers which can further associate to tetramers. Interacts with isoform 1 of ACP1. SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. PTM: Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro (By similarity). SIMILARITY: SwissProt: Q13813 ## Belongs to the spectrin family. & Contains 3 EF-hand domains. & Contains 1 SH3 domain. & Contains 23 spectrin repeats. |
| Molecular Weight | ~240 kDa full length product, ~150 kDa N-terminal cleaved product and ~120 kDa C-terminal product may be seen in varing amounts in lysates, depending on condition. The smaller sizes represent break down products seen during apoptosis. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot on staurosporine-treated HeLa lysates. Western Blot Analysis: 1:500 dilution of this antibody detected N-, C- terminal Spectrin alpha/Fodrin alpha on 10 μg of staurosporine treated HeLa lysates. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 12 months at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |