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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, Fe | RIA, WB, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1628 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Myosin Antibody, slow muscle, clone NOQ7.5.4D |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Liquid. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Myosin Antibody, slow muscle, clone NOQ7.5.4D is an antibody against Myosin for use in RIA, WB, IH. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: frozen and formalin fixed sections. Immunoblotting RIA Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Myosin purified from myofibrils isolated from histochemically mixed human skeletal muscle. |
| Epitope | slow muscle |
| Clone | NOQ7.5.4D |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Slow myosin heavy chain. Clearly identifies Type 1 fibers. Within skeletal muscle MAB1628 is specific for slow myosin heavy chain in a wide variety of species. It reacts strongly with rat and feline slow myosin heavy chain. MAB1628 also identifies beta (slow) myosin heavy chain in heart ventricles. |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | MYH7 encodes the cardiac muscle beta (or slow) isoform of myosin. Changes in the relative abundance of MYH7 and MYH6 (the alpha, or fast, isoform of cardiac myosin heavy chain) correlate with the contractile velocity of cardiac muscle. Mutations in MYH7 are associated with familial hypertrophic cardiomyopathy. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P12883 # Muscle contraction. SIZE: 1935 amino acids; 223097 Da SUBUNIT: Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). SUBCELLULAR LOCATION: Cytoplasm, myofibril. Note=Thick filaments of the myofibrils. DOMAIN: SwissProt: P12883 The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils. DISEASE: SwissProt: P12883 # Defects in MYH7 are the cause of familial hypertrophic cardiomyopathy type 1 (CMH1) [MIM:192600]; also designated FHC or HCM. Hypertrophic cardiomyopathy is a heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The prevalence of the disease in the general population is 0.2%. FHC is clinically heterogeneous, with inter- and intrafamilial variations ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. & Defects in MYH7 are the cause of myosin storage myopathy [MIM:608358]. In this disorder, muscle biopsy shows type 1 fiber predominance and increased interstitial fat and connective tissue. Inclusion bodies consisting of the beta cardiac myosin heavy chain are present in the majority of type 1 fibers, but not in type 2 fibers. & Defects in MYH7 are a cause of dilated cardiomyopathy (CMD) [MIM:115200]. CMD is a disorder characterized by cardiac dilation and reduced systolic function. & Defects in MYH7 are the cause of Laing early-onset distal myopathy (MPD1) [MIM:160500]. MPD1 is an autosomal dominant disorder which differs from other distal myopathies in that onset is as early as 4 years of age. Selective weakness of the anterior tibial muscles is followed by weakness of the finger extensors and selected proximal muscle groups such as the hip abductors and rotators, the shoulder abductors and the sternocleidomastoids. SIMILARITY: Contains 1 IQ domain. & Contains 1 myosin head-like domain. MISCELLANEOUS: Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2). & The cardiac alpha isoform is a fast ATPase myosin, while the beta isoform is a slow ATPase. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |