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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Rb | IP, WB, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1676 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Talin 1 Antibody, NT, a.a. 139-433, clone TA205 |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Liquid in 0.02 M PB pH 7.6, 0.25 M NaCl containing 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Talin 1 Antibody, N-terminus, a.a. 139-433, clone TA205 detects level of Talin & has been published & validated for use in IP, WB, IH. |
| Key Applications |
|
| Application Notes | Immunoblotting, recognizes a band at ~230kDa. Immunoprecipitation Immunofluorescence Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Human platelet protein |
| Epitope | N-terminus, a.a. 139-433 |
| Clone | TA205 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Human Talin. This antibody reacts with an N-terminal epitope in talin, between amino acids 139-433. On microinjection into whole cells MAB1676 disrupts actin stress fibers and focal contacts and inhibits cell motility. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a cytoskeletal protein which is concentrated in areas of cell-substratum and cell-cell contacts. This protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin. |
| Gene Symbol |
|
| Non-Reactive Species |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q9Y490 # Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts (By similarity). SIZE: 2541 amino acids; 269767 Da SUBUNIT: Binds with high affinity to vinculin and with low affinity to integrins. Interacts with PIP5K1C and NRAP. Interacts with LAYN. SUBCELLULAR LOCATION: Cell projection, ruffle membrane (By similarity). Note=Colocalizes with LAYN at the membrane ruffles (By similarity). SIMILARITY: SwissProt: Q9Y490 ## Contains 1 FERM domain. & Contains 1 I/LWEQ domain. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain between 2 and 8°C for up to six months. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |