Cayman Thioredoxin Reductase Colorimetric Assay Kit; Size- 96 Wel

Cayman’s Thioredoxin Reductase (TrxR) Assay Kit provides a convenient method for quantifying mammalian TrxR activity from tissue homogenates and cell lysates in a 96-well plate format. In this assay, TrxR uses NADPH to reduce DTNB to 5-thio-2-nitrobenzoic acid (TNB) which absorbs strongly at 405-414 nm.^, The kit includes all reagents needed to assay mammalian TrxR activity. Measurement of TrxR activity in the absence and in the presence of aurothiomalate, a specific TrxR inhibitor included in the kit, allows for correction of non-thioredoxin reductase-independent DTNB reduction.

Needed but not supplied:Please to verify if (Milli-Q or equivalent) or any other components are needed for this assay.

WARNING This product is not for human or veterinary use.