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Cayman Brd4 Bromodomains 1 And 2 (Human Recombinant; Aa 2-477)
NETA PART: | CAYM-14822-1 |
MFG.PART: | 14822-1 |
UNSPSC: | 12352200 |
Manufacturer: | Cayman |
Product Description
Bromodomain-containing protein 4 (BRD4) is a member of the bromodomain and extra-terminal domain (BET) family.1 It is a ubiquitously expressed nuclear protein with roles in a variety of cellular processes, including regulation of gene transcription, cell cycle progression, and viral genome segregation.1,2 BRD4 is comprised of two N-terminal bromodomains (BD1 and BD2) that can bind to acetylated lysine residues in histones, serving to couple histone acetylation marks to the transcriptional regulation of target promoters, an extra-terminal (ET) domain that facilitates protein-protein interactions, and a C-terminal motif, and can be expressed as either a long or short isoform generated via alternative splicing.1,2,3 In addition to binding acetylated lysine residues on histones, BRD4 can bind to a variety of non-histone proteins and protein complexes, including positive transcription elongation factor b (P-TEFb) and the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) envelope (E) protein, a transmembrane protein involved in CoV virion assembly and pathogenesis of the related virus, SARS-CoV.1,4,5,6 In mice, knock-down of Brd4 is embryonic lethal.2 Chromosomal translocations leading to the fusion of BRD4 with the nuclear protein in testis (NUT) gene and resulting in expression of BRD4-NUT fusion proteins are associated with NUT midline carcinoma (NMC).1,3 Inhibition of BRD4-NUT binding to chromatin by the BET bromodomain inhibitor (+)-JQ1 (Item No. 11187) induces tumor regression and prolongs survival in NMC mouse xenograft models.7 Cayman’s BRD4 bromodomains 1 and 2 (human, recombinant; aa 2-477) protein can be used for ELISA, Western blot (WB), and binding assay applications.
WARNING This product is not for human or veterinary use.
Technical Information
Synonyms |
|
Purity | ≥70% estimated by SDS-PAGE |
Source | Recombinant N-terminal GST-tagged protein expressed inE. Coli |
Amino Acids | 2-477 (partial protein) |
MW | 80.6 kDa |
Formulation(Request formulation change) | 10 mM HEPES, pH 7.5, with 500 mM sodium chloride, and 5% glycerol |
UniProt Accession № | O60885 |
Storage | -80°C |
Shipping | Dry ice in continental US; may vary elsewhere |
Stability | ≥ 2 years |
SKU | CAYM-14822-1 |
---|---|
Supplier Part Number | 14822-1 |
UM | EA |
UNSPSC | 12352200 |
Manufacturer | Cayman |
MSDS URL | https://www.caymanchem.com/msdss/14822m.pdf |
Temperature | -80 |
Refrigerated-Frozen | Frozen |
CountryOfOrigin | US |
ProductLine | CAYM |
Qty | 1 |
MinOrderQty | 1 |
Weight | 7.00 |
Lead Time | 2 Business Days |
Hazardous | N |