Cayman His-Tag Rabbit Monoclonal Antibodybiotinylated, 50 G

His-tag Monoclonal Antibody - Biotinylated is a probe for the immunochemical detection of histidine tags on recombinant proteins. Recombinant proteins are commonly labeled with affinity tags, such as 6-10 histidine residues (6x-10x His), to facilitate both their detection and purification. Poly-His tags are commonly utilized because of their small size, low potential to interfere in protein folding or activity, weak immunogenicity, and high affinity for transition metal ion matrices, such as Ni²⁺, used in immobilized metal-affinity chromatography (IMAC) for protein purification.^,. Caymans His-tag Rabbit Monoclonal Antibody - Biotinylated Clone RM146) can be used for ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunohistochemistry (IHC), and Western blot (WB) applications. The antibody recognizes proteins containing 6x or 10x His tags fused to either the N- or C-terminus.

WARNING This product is not for human or veterinary use.