Cayman Sphk1 Assay Buffer (1x); Size- 14 Ml

This polyclonal antibody is labeled with R-Phycoerythrin (PE) and can be used for immunofluorescent labeling of cellular EP₂ receptor following permeabilization. R-PE absorption maxima occurs at 565>540>498 nm and the emission maximum occurs at 578 nm, however standard excitation by 488 nm lasers is sufficient to generate signal detected in the PE channel of flow cytometers. The biological effects of prostaglandin E₂ (PGE₂) are mediated through interaction with four distinct membrane-bound G-protein coupled EP receptors: EP₁, EP₂, EP₃, and EP₄.^, Binding of PGE₂ to the EP₂ receptor results in an increase in adenylate cyclase activity with a subsequent increase in cAMP.^, Pharmacologically, EP₂ receptors mediate relaxation of smooth muscle and are distinguished from EP₄ receptors by their sensitivity to the EP₂ receptor selective agonist butaprost.^,, The human EP₂ receptor is comprised of 358 amino acids with a molecular mass of approximately 40,000. mRNA for the EP₂ receptor is expressed in a variety of tissues including lung, placenta, spleen, intestine, kidney, and sensory neuron.^,,

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