Agilent Technologies Advancebio Sialidase V, Recombinant 1 U

Agilent Technologies specifications: - concentration: 5 u/mL. Enzyme applications: glycan analysis, glycan digestion, sialic acid analysis, sialic acid removal, desialylation. Enzyme formulation: sterile-filtered solution in 50 mm sodium acetate, 150 mm nacl, ph 5.5. Enzyme source: recombinant, expressed in e. Coli, from vibrio cholerae. Enzyme specificity: cleaves all nonreducing terminal alpha (2-3,6,8)-linked-sialic acid from oligosaccharides, glycoproteins, and complex carbohydrates. Enzyme unit definition: one unit of advancebio sialidase v is defined as the amount of enzyme required to catalyze the release of 1 umol of methylumbelliferone from mu-nana [2-(4-methylumbelliferyl) - alpha -d-n-acetylneuraminic acid] per minute at ph 5.5 and 37 c. Storage temp conditions: refrigeration required, 2-8 c. Volume: 200 ul. - BC