AG Scientific Dithiothreitol [DTT], 10 G

Description:

Dithiothreitol (DTT), is a small-molecule redox reagent, known as Clelands reagent, that forms a six-member ring with an internal disulfide bond. It has a redox potential of -0.33 V at pH 7. The reduction of typical disulfide bond proceeds by two sequential thiol-disulfide exchange reactions.

DTT is effective in buffers to reduce protein disulfide bonds prior to SDS-PAGE. It can protect protein sulfhydryls and restore enzyme activity lost by oxidation.

Applications:

• For reducing or "deprotecting" agent for thiolated DNA
• For reducing disulfide bonds of proteins
• Used as an oxidizing agent
• Used as a component of medium for the demembranation and reactivation of spermatozoa
• Used to prevent intramolecular and intermolecular disulfide bonds from forming between cysteine residues of proteins

Additional Reading from AG Scientific:

• DTT (Dithiothreitol): Frequently Asked Questions
• DTT (Dithiothreitol): Applications You Must Know
• TCEP HCl vs. DTT: Preferred Reducing Agents
• Download Brochure: Reducing Agents