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Cayman EZ Cap™ Firefly Luciferase mRNA (mo5U)
NETA PART: | CAYM-39801-1 |
MFG.PART: | 39801-1 |
UNSPSC: | 12352202 |
Manufacturer: | Cayman |
Product Description
The constitutive androstane receptor (CAR), also known as NR1I3 (nuclear receptor subfamily 1, group I, member 3), is a nuclear hormone receptor with activity similar to that seen in other steroid receptors such as estrogen or progesterone but more similar in form to PPAR, LXR and RXR. CAR functions differently from other steroid receptors and its activity is still being elucidated.It is known to act in concert with PXR to detoxify xenobiotics. CAR is encoded by the NR1I3 gene. This gene encodes a member of the nuclear receptor superfamily, and is a key regulator of xenobiotic and endobiotic metabolism. The protein binds to DNA as a monomer or a heterodimer with the retinoid X receptor and regulates the transcription of target genes involved in drug metabolism and bilirubin clearance, such as cytochrome P450 family members. Unlike most nuclear receptors, this transcriptional regulator is constitutively active in the absence of ligand but is regulated by both agonists and inverse agonists. Ligand binding results in translocation of this protein to the nucleus, where it activates or represses target gene transcription. These ligands include bilirubin, a variety of foreign compounds, steroid hormones, and prescription drugs. Multiple transcript variants encoding different isoforms have been found for this gene. CAR exists as three predominant splice variants in humans depicted as CAR-1, CAR-2 and CAR-3. Each has unique biological properties. These splice variants do not exist in rat and mouse.Unlike CAR-1, CAR-2 is not constitutively active, showing ligand-dependent activation of reporter genes linked to genetic response elements derived from CYP2B6 or CYP3A4 promoters. Diethylhexyl phthalate (DEHP) is a strong agonist of CAR-2 but has no activity toward CAR-1 or CAR-3.It is noteworthy, and a source of experimental confusion, that a number of xenobiotics characterized as activators of human CAR (including phenobarbital) actually modulate the receptors activity via indirect mechanisms. In other words, such chemicals do not directly bind to CAR; rather, they impact the activity of upstream regulatory mechanisms that impinge on CAR activity. Hybrid nuclear receptors in which the native N-terminal DNA binding domain (DBD) has been substituted with the GAL4 DBD, such as is used in this reporter assay system, likely will not be responsive to chemical modulators that act through indirect mechanisms.CAR-2 Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments. [INDIGO Catalog Nos. IB00921-32, IB00921, IB00922]
WARNING This product is not for human or veterinary use.
Technical Information
- CAR2
- NR1I3i2
Shipping & Storage Information
SKU | CAYM-39801-1 |
---|---|
Supplier Part Number | 39801-1 |
UM | EA |
UNSPSC | 12352202 |
Manufacturer | Cayman |
Temperature | -80 |
Refrigerated-Frozen | Frozen |
CountryOfOrigin | US |
ProductLine | CAYM |
Qty | 1 |
MinOrderQty | 1 |
Weight | 7.00 |
Lead Time | 2 Business Days |
Hazardous | N |