Cayman Prostaglandin E2 TR-FRET Assay Kit

Cayman’s Prostaglandin E₂ (PGE₂) TR-FRET Assay Kit provides a robust and easy-to-use time-resolved Förster resonance energy transfer (TR-FRET) platform for measurement of PGE₂ in cell culture supernatants. The assay utilizes a PGE₂ monoclonal antibody directly labeled with a europium (Eu³⁺) chelate as a donor molecule and fluorescently labeled PGE₂ as the acceptor molecule. In the absence of free PGE₂, the acceptor-labeled PGE₂ is bound to the europium-labeled PGE₂ antibody resulting in FRET from the donor to the acceptor upon excitation of the Eu³⁺ chelate at 340 nm and an emission from the acceptor at 665 nm. If free PGE₂ is present, it will compete with the PGE₂ acceptor for binding to the donor-labeled PGE₂ antibody, causing a decrease in the TR-FRET signal. The specific signal at 665 nm will be inversely proportional to the PGE₂ concentration in the sample. Data can be expressed as either the signal at 665 nm or the 665/615 nm ratio. This assay has a range of 0.038-600 ng/ml with lower limit of detection of approximately 0.16 ng/ml.

Needed but not supplied:Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

WARNING This product is not for human or veterinary use.