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Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application
Recommended Usage
Competitive ELISA
1-30 μg/ml
Sandwich ELISA
0.5-2 μg/ml
ELISA
0.01-1 µg/ml
Properties
Form
Lyophilized
Storage Buffer
Lyophilized with PBS, pH 7.2, containing 0.02% sodium azide.
Reconstitution
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
Storage Instructions
The lyophilized product remains stable for up to 1 year at -20 °C from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Purification
Protein A affinity column
Isotype
Rabbit IgG, κ
Clonality
Monoclonal
Clone ID
12A3
Note
GenScript can customize this product per customers request including product size, buffer components, etc.
Background
Target Background
Certolizumab pegol, marketed as Cimzia, is a biopharmaceutical drug used to treat Crohns disease, rheumatoid arthritis, psoriatic arthritis, and ankylosing spondylitis. It is a medication that consists of a humanized Fab fragment, derived from an IgG1 antibody, combined with a polyethylene glycol moiety. This modification replaces the Fc region of the antibody. The purpose of removing the Fc region is to prevent complement fixation and antibody-mediated cytotoxicity, while also significantly extending the half-life of the medication.