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The product is specific to Biotin and biotinylated antibody, protein, peptides, oligonucleotides, or solid matrices.
Host Species
Rabbit
Conjugate
PE
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application
Recommended Usage
Immunocytochemistry/Immunofluorescence (ICC/IF)
1:100-1:500
Flow Cytometry
1:100-1:500
Properties
Form
Liquid
Storage Buffer
Supplied in PBS (pH 7.2), containing gelatin and 0.03% ProClin300.
Concentration
0.5 mg/ml
Storage Instructions
Stored at 4°C and it should be protected from prolonged exposure to light. Do not freeze. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification
Protein A/G affinity column
Isotype
Rabbit IgG
Clonality
Monoclonal
Clone ID
53C8
Note
GenScript can customize this product per customers request including product size, buffer components, etc.
Background
Target Background
Biotin is widely conjugated to proteins and antibodies for biochemical assays. Avidin (streptavidin)-biotin system is commonly used for many immunoassays such as ELISA, Flow Cytometry, Immunofluorescence, In Situ Hybridization, and Immunohistochemistry. Anti-Biotin mAb is a better alternative to avidins to minimize background and maximize signal intensity.