RPI Zymo-Seq miRNA Library Kit, 12 preps

RPI The Zymo-Seq miRNA Library Kit is an innovative small RNA sequencing library prep kit that uses a single-adapter and circularization strategy to reduce ligation bias and provide accurate small RNA profiling from cell-free RNA (cfRNA) and total RNA. This simple, streamlined workflow minimizes hands-on time and uses gel-free removal of adapter dimers to generate stranded small RNA libraries from plasma, biofluids, cells, tissues, and more.To prepare Zymo-Seq miRNA libraries, RNA is ligated to the single miRNA adapter and the ligated product is circularized. Excess adapter is blocked and removed with specialized magnetic beads that allow a completely gel-free workflow for input as low as 1 ng. The circularized miRNA-adapter product is then reverse transcribed. The resulting cDNA is amplified using Indexing PCR primers which add on Illumina-compatible adapter and unique dual index (UDI) sequences for cost-effective multiplexing. Everything needed to make sequencing-ready libraries, including indexing primers and SPRI beads, are included in the kit. Its RNA-Seq made simple.Accurate miRNA profiling: A unique single adapter and circularization strategy reduces ligation bias and increases miRNA detection.Direct detection: Generates small RNA libraries without the need for small RNA enrichment.Gel-Free: Removes adapter dimers from any input without lengthy gel excision. Storage Temperature: -20C Additional Components Required (Not Provided): Molecular biology grade pure ethanol (> 95%)Maximum Input: 200 ng of total RNA, or RNA extracted from 200 L of plasma or serum.Minimum Input: 1 ng of total RNA, or RNA extracted from 50 L of plasma or serum.Processing Time: 2 hours hands-on, 8 hours totalRecommended Equipment: A 96-well Aluminum Cooler Block for PCR tubes or a 96-well PCR tube rack may be placed on ice for ease of reagent assembly and maintaining samples at 4C. To process > 12 samples at once, a multichannel pipette with low-retention tips is recommended for mixing and the timely addition of pre-aliquoted reagents.Recommended Input: 100 ng of total RNA, or RNA extracted from 200 L of plasma or serum.Required Equipment: Thermal cycler, magnetic stand for 0.2 mL PCR tubes or 96-well plate, mini centrifuge, and micro centrifuge.Sample Type: High integrity total RNA (RIN > 7) containing miRNA, or cell-free RNA extracted from plasma/serum samples not preserved with heparin. Small RNA enrichment is not necessary.Sequencing Platform: Compatibility Libraries are compatible with all Illumina sequencing platforms.Shipping Weight: 4.0001 lbs Research or further manufacturing use only, not for food or drug use. - RPI