We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
Share this :
Print
RPI Zymo-Seq miRNA Library Kit, 96 preps
RPI Zymo-Seq Mirna Library Kit, 96 Preps - RPI
List Price
$7,874.35
Your Price
$7,874.35
HOW MUCH YOU SAVE:
0.00 %
NETA PART: | RPI-ZR3007 |
MFG.PART: | ZR3007 |
UNSPSC: | 41116133 |
Manufacturer: | RPI |
RPI The Zymo-Seq miRNA Library Kit is an innovative small RNA sequencing library prep kit that uses a single-adapter and circularization strategy to reduce ligation bias and provide accurate small RNA profiling from cell-free RNA (cfRNA) and total RNA. This simple, streamlined workflow minimizes hands-on time and uses gel-free removal of adapter dimers to generate stranded small RNA libraries from plasma, biofluids, cells, tissues, and more.To prepare Zymo-Seq miRNA libraries, RNA is ligated to the single miRNA adapter and the ligated product is circularized. Excess adapter is blocked and removed with specialized magnetic beads that allow a completely gel-free workflow for input as low as 1 ng. The circularized miRNA-adapter product is then reverse transcribed. The resulting cDNA is amplified using Indexing PCR primers which add on Illumina-compatible adapter and unique dual index (UDI) sequences for cost-effective multiplexing. Everything needed to make sequencing-ready libraries, including indexing primers and SPRI beads, are included in the kit. Its RNA-Seq made simple.Accurate miRNA profiling: A unique single adapter and circularization strategy reduces ligation bias and increases miRNA detection.Direct detection: Generates small RNA libraries without the need for small RNA enrichment.Gel-Free: Removes adapter dimers from any input without lengthy gel excision. Storage Temperature: -20C Additional Components Required (Not Provided): Molecular biology grade pure ethanol (> 95%)Maximum Input: 200 ng of total RNA, or RNA extracted from 200 L of plasma or serum.Minimum Input: 1 ng of total RNA, or RNA extracted from 50 L of plasma or serum.Processing Time: 2 hours hands-on, 8 hours totalRecommended Equipment: A 96-well Aluminum Cooler Block for PCR tubes or a 96-well PCR tube rack may be placed on ice for ease of reagent assembly and maintaining samples at 4C. To process > 12 samples at once, a multichannel pipette with low-retention tips is recommended for mixing and the timely addition of pre-aliquoted reagents.Recommended Input: 100 ng of total RNA, or RNA extracted from 200 L of plasma or serum.Required Equipment: Thermal cycler, magnetic stand for 0.2 mL PCR tubes or 96-well plate, mini centrifuge, and micro centrifuge.Sample Type: High integrity total RNA (RIN > 7) containing miRNA, or cell-free RNA extracted from plasma/serum samples not preserved with heparin. Small RNA enrichment is not necessary.Sequencing Platform: Compatibility Libraries are compatible with all Illumina sequencing platforms.Shipping Weight: 10.0002 lbs Research or further manufacturing use only, not for food or drug use. - RPI
SKU | RPI-ZR3007 |
---|---|
Supplier Part Number | ZR3007 |
UM | CS |
UNSPSC | 41116133 |
Manufacturer | RPI |
ProductLine | RPI |
Qty | 1 |
MinOrderQty | 1 |
Weight | 7.00 |
Lead Time | 5 Business Days |
Hazardous | N |