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Cell Signaling Dna-Pkcs Antibody
Cell Signaling Dna-Pkcs Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)
List Price
$129.00
Your Price
$129.00
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NETA PART: | CSIG-4602T |
MFG.PART: | 4602T |
UNSPSC: | 41116111 |
Manufacturer: | Cell Signaling |
Size | 20 µl |
Reactivity | H |
Sensitivity | Endogenous |
Molecular Weight (kDa) | 450 |
Source/Isotype | Rabbit |
Application/Dilution | {Western Blotting: 1:1000} |
Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
Specificity/Sensitivity | DNA-PKcs Antibody detects endogenous levels of DNA-PKcs protein. |
Species Reactivity | Human |
Source/Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids near the carboxy-terminus of human DNA-PKcs. Antibodies are purified by protein A and peptide affinity chromatography. |
Background | DNA-dependent protein kinase (DNA-PK) is an important factor in the repair of double-stranded breaks in DNA. Cells lacking DNA-PK or in which DNA-PK is inhibited fail to show proper nonhomologous end-joining (NHEJ) (1-7). DNA-PK is composed of two DNA-binding subunits (Ku70 and Ku86) and one 450 kDa catalytic subunit (DNA-PKcs) (8). It is thought that a heterodimer of Ku70 and Ku86 binds to double-stranded DNA broken ends before DNA-PKcs binds and is activated (1,9). Activated DNA-PKcs is a serine/threonine kinase that has been shown to phosphorylate a number of proteins in vitro, including p53, transcription factors, RNA polymerase, and Ku70/Ku86 (10,11). DNA-PKcs autophosphorylation at multiple sites, including Thr2609 and Ser2056, results in an inactivation of DNA-PK kinase activity and NHEJ ability (12,13). It has been demonstrated, however, that DNA-PK preferentially phosphorylates substrates before it autophosphorylates, suggesting that DNA-PK autophosphorylation may play a role in disassembly of the DNA repair machinery (14,15). Autophosphorylation at Thr2609 has also been shown to be required for DNA-PK-mediated double-strand break repair, and phosphorylated DNA-PK co-localizes with H2A.X and 53BP1 at sites of DNA damage (16). Phosphorylation at Ser2056 occurs in response to double-stranded DNA breaks and ATM activation (17). |
SKU | CSIG-4602T |
---|---|
Supplier Part Number | 4602T |
UM | UNIT |
UNSPSC | 41116111 |
Manufacturer | Cell Signaling |
MSDS URL | https://www.cellsignal.com/contents/technical/safety-data-sheet-(sds)/resources-safety-data-sheets |
Temperature | -20C |
ProductLine | CSIG |
Qty | 1 |
MinOrderQty | 1 |
Weight | 7.00 |
Lead Time | 5 Business Days |