Special Offers
100% Performance Guaranteed
Key Specifications Table
Species Reactivity | Key Applications | Host | Format | Antibody Type |
---|---|---|---|---|
H, M | WB | Rb | Affinity Purified | Polyclonal Antibody |
Description | |
---|---|
Catalogue Number | ABE251 |
Replaces | 06-892 |
Description | Anti-N-CoR Antibody |
Alternate Names |
|
Background Information | In the absence of ligands, the corepressor N-CoR represses nuclear hormone receptor-mediated gene transcription. In the presence of ligands, these hormone receptors activate gene expression and play critical roles in development, growth, and homeostasis. N-Cor-mediated transcriptional repression is facilitated through the formation of histone deacetylase (HDACs) N-CoR complexes or by conjugation from small ubiquitin-like modifier 1 (SUMO-1). It is shown that the SANT1 domain of N-CoR serves as a binding region for SUMO-E2 conjugating enzyme Ubc9 and SUMO-E3 ligase Pias1, which is necessary for N-CoR-mediated repression of respective reporter genes. SUMOylation-deficient N-COR knockout mice display a signification reduction in the repression of gene transcription and result in defects involving the differentiation and development of neural cells into erythrocytes and thymocytes. |
Product Information | |
---|---|
Format | Affinity Purified |
Control |
|
Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
Quality Level | MQ100 |
Applications | |
---|---|
Application | Anti-N-CoR Antibody is a Rabbit Polyclonal Antibody for detection of N-CoR also known as Nuclear receptor corepressor 1 & has been validated in WB. |
Key Applications |
|
Biological Information | |
---|---|
Immunogen | KLH-conjugated linear peptide corresponding to a region between amino acids 1510 and 1540 of the N-CoR protein. |
Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
Host | Rabbit |
Species Reactivity |
|
Antibody Type | Polyclonal Antibody |
Entrez Gene Number |
|
Gene Symbol |
|
Purification Method | Affinity Purfied |
UniProt Number |
|
UniProt Summary | FUNCTION: Mediates transcriptional repression by certain nuclear receptors. Part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors. SUBUNIT STRUCTURE: Interacts with ZBTB33; the interaction serves to recruit the N-CoR complex to promoter regions containing methylated CpG dinucleotides. Interacts with TRIM28 and KDM3A. Interacts (via the RD1 domain) with BAZ1A (via its N-terminal); the interaction corepresses a number of NCOR1-regulated genes. Interacts with HEXIM1 By similarity. Interacts with C1D, SIAH2, HDAC7, SAP30, SIN3A and SIN3B. Forms a large corepressor complex that contains SIN3A/B and histone deacetylases HDAC1 and HDAC2. This complex associates with the thyroid receptor (TR) and the retinoid acid receptor (RAR) in the absence of ligand. Interacts directly with RARA; the interaction is facilitated with RARA trimethylation. Interacts with DACH1. Component of the N-Cor repressor complex, at least composed of CBFA2T3, HEXIM1, NCOR1, NCOR2, HDAC3, TBL1X, TBL1XR1, CORO2A and GPS2. SUBCELLULAR LOCATION: Nucleus. |
Molecular Weight | ~270 kDa observed. Uniprot describes 2 isoforms produced by alternative splicing at MW of 266 kDa and 270 kDa. |
Product Usage Statements | |
---|---|
Quality Assurance | Evaluated by Western Blot in RAW 264.7 cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected N-CoR in RAW 264.7 cell lysate. No tween for best results. |
Usage Statement |
|
Storage and Shipping Information | |
---|---|
Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
Packaging Information | |
---|---|
Material Size | 100 µg |