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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, H, M | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN60 |
| Description | Anti-Tryptophan hydroxylase 2 Antibody |
| Alternate Names |
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| Background Information | Tryptophan hydroxylase 2 (TPH2) is preferentially expressed in the brain and is thought to play an important role in serotonin synthesis. Recent research has actually associated high levels of TPH2 with risk of suicide. Misfolding of TPH2 may be the cause of a loss of serotonin neuronal function in Parkinson’s disease. Detection and screening of particular mutations in TPH2 has been cited as an approach toward predicting patient sensitivity to potential antidepressant therapies and even the possibility of diagnosis of behavioral disorders. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
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| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Tryptophan hydroxylase 2 Antibody detects level of Tryptophan hydroxylase 2 & has been published & validated for use in WB, IH(P). |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: 1:400 dilution from a previous lot detected Tryptophan hydroxylase 2 in rat cerebral cortex tissue. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human Tryptophan hydroxylase 2. |
| Host | Rabbit |
| Species Reactivity |
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| Species Reactivity Note | Demonstrated to react with rat and human. Predicted to react with mouse based on immunogen design. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | CATALYTIC ACTIVITY: L-tryptophan + tetrahydrobiopterin + O2 = 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin. COFACTOR: Fe2+ ion By similarity. PATHWAY: Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2. TISSUE SPECIFICITY: Brain specific. INVOLVEMENT IN DISEASE: Genetic variation in TPH2 may influence susceptibility to major depressive disorder (MDD) [MIM:608516]. Defects in TPH2 are the cause of susceptibility to attention deficit-hyperactivity disorder type 7 (ADHD7) [MIM:613003]. ADHD is a neurobehavioral developmental disorder and is primarily characterized by the co-existence of attentional problems and hyperactivity, with each behavior occurring infrequently alone. Note=Naturally occurring variants of TPH2 with impaired enzyme activity could cause deficiency of serotonin production and result in an increased risk of developing behavioral disorders. SEQUENCE SIMILARITIES: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. Contains 1 ACT domain. BIOPHYSICOHEMICAL PROPERTIES: Kinetic parameters: KM=41.3 µM for L-tryptophan Vmax=833 nmol/min/mg enzyme |
| Molecular Weight | ~56 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in rat brain tissue lysate. Western Blotting Analysis: 0.2 µg/mL of this antibody detected Tryptophan hydroxylase 2 on 10 µg of rat brain tissue lysate. |
| Usage Statement |
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| Packaging Information | |
|---|---|
| Material Size | 100 µg |