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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R | ICC, IHC | Rb | AlexaFluor®488 | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN78A4 |
| Description | Anti-NeuN (rabbit) Antibody, Alexa Fluor® 488 Conjugate |
| Alternate Names |
|
| Background Information | ABN78 is a rabbit polyclonal version of the Anti-NeuN, clone A60 (MAB377), NeuN is a highly characterized and cited mouse monoclonal antibody that specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found. |
| Product Information | |
|---|---|
| Format | AlexaFluor®488 |
| Control |
|
| Presentation | Purified rabbit polyclonal conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect NeuN (rabbit) using this Anti-NeuN (rabbit) Antibody, Alexa Fluor 488 Conjugate validated for use in IC, IH. |
| Key Applications |
|
| Application Notes | Immunocytochemistry analysis: A 1:100 dilution from a representative lot detected NeuN in rat E18 primary cortex cells. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to mouse NeuN. |
| Epitope | N-terminus |
| Host | Rabbit |
| Specificity | This antibody recognizes NeuN at the N-terminus. |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Mouse and Rat. The unconjugated rabbit Anti-NeuN (Catalog No. ABN78) has been shown to work in human. |
| Antibody Type | Polyclonal Antibody |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| Molecular Weight | The unconjugated parent antibody (Cat. No. ABN78) has an observed molecular weight at ~46/48 kDa. An uncharacterized band appears at ~62 kDa in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in adult mouse brain tissue. Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected NeuN in adult mouse brain tissue. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |