Key Specifications Table
| Source | Purity |
|---|---|
| E. coli. | Product is endotoxin free (< 0.1ng/μg of protein (<1EU/μg) and greater than 98% pure by SDS-PAGE gel and HPLC analyses |
| Description | |
|---|---|
| Catalogue Number | GF174 |
| Description | Sonic HedgeHog (Shh) Protein, Human Recombinant |
| Alternate Names |
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| Background Information | The hedgehog (hh) gene encoding a secreted protein was originally identified in Drosophila as a segment polarity gene. The vertebrate homologues of hh comprise several proteins including sonic hedgehog (Shh), Indian hedgehog (Ihh), and Desert hedgehog (Dhh). Hedgehog proteins are important signaling molecules during embryonic development. Shh is expressed in key embryonic tissues such as the Hensen’s node, the zone of polarizing activity in the posterior limb bud, the notochord, and the floor plate of the neural tube. Shh is involved in regulating the patterning of the developing central nervous system, somite, and limb. Shh plays an important role in the development of particular tissues such as whisker, hair, foregut, tooth and bone. Evidence also suggests that Shh is involved in regulating stem cell fates of neural and hematopoietic lineages, and that aberrant Shh signaling is implicated in basal cell carcinomas and other diseases. Shh genes are highly conserved and have been identified in a variety of species including human, mouse, frog, fish, and chicken. Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues, including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue. |
| Product Information | |
|---|---|
| Presentation | Product is filtered through a 0.2 micron filter before lyophilization. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | The hedgehog (hh) gene encoding a secreted protein was originally identified in Drosophila as a segment polarity gene. |
| Biological Information | |
|---|---|
| Purity | Product is endotoxin free (< 0.1ng/μg of protein (<1EU/μg) and greater than 98% pure by SDS-PAGE gel and HPLC analyses |
| Source | E. coli. |
| Cross Reactivity | None |
| UniProt Number |
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| Product Usage Statements | |
|---|---|
| Quality Assurance | Bioactivity assay: Determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) cells. The expected ED50 for this effect is 0.8-1.0 μg/ml. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Store at -20°C for up to 6 months from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 µg |