Takara NucleoSpin cfDNA Midi Core Kit \48 Preps

Takara The NucleoSpin cfDNA Midi Core Kit includes all of the components provided with the NucleoSpin cfDNA Midi kit (Cat. # 740303.48) minus the 24-Square-well Block 10 mL, and is designed for the rapid, medium-scale preparation of highly pure cell-free DNA from up to 5 ml of blood plasma using vacuum filtration. The purification protocol involves lysis of plasma samples using Proteinase K incubation in the presence of a chaotropic salt-containing buffer. Following the initial lysis step, a binding buffer is added to the samples and the lysate is loaded onto the binding columns. After loading and vacuum filtration-driven binding of DNA to the silica membrane, impurities are removed by several washing steps and cfDNA is eluted after a final drying step. The use of vacuum filtration technology eliminates tedious centrifugation steps and manual handling of columns during processing. The NucleoSpin cfDNA Midi Core Kit allows for processing of up to 24 samples in parallel in 90 min. - TAKBGN (Additional S&H or Hazmat Fees May Apply)