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Genscript CHO-K1/mouse CTLA4 Stable Cell Line
Genscript CHO-K1/mouse CTLA4 Stable Cell Line - GSCRPT (Additional S&H or Hazmat Fees May Apply)
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$8,925.00
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$8,925.00
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| NETA PART: | GSCRPT-M00570 |
| MFG.PART: | M00570 |
| UNSPSC: | 41106514 |
| Manufacturer: | GenScript USA Inc |
Overview
| Product Description | Recombinant CHO-K1 cells stably overexpress Mus musculus cytotoxic T-lymphocyte-associated protein 4 (CTLA4) on the surface. The surface expression of mouse CTLA4 is validated by FACS analysis. This cell line is designed for cell-based binding for screening antibodies binding with mouse CTLA4 and for evaluating target binding affinity. |
| Applications | Cell-based binding assay or use as immunogen |
Product Introduction
| Expressed Gene | Codon optimized from NM_009843.4; no expressed tags |
| Target Protein | NP_033973 |
| Host Cell | CHO-K1 |
| Quantity | 2 vials of frozen cells (1x106 per vial in 1 mL) |
| Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status | Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No.LT07-318, Lonza). |
| Stability | Stable through more than 15 passages with no significant changes in assay performance or expression profile. |
Culture Conditions
| Culture Properties | Adherent |
| Freeze Medium | 95% Culture Medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma) |
| Complete Growth Medium | Ham’s F-12K (Kaighn’s) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco) |
| Culture Medium | Ham’s F-12K (Kaighn’s) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco), 8 μg/ml Puromycin (Cat. No. A11138-03, Gibco) |
Background
| Gene Synonyms | Cd152; Ly-56; Ctla-4 |
| Background | CTLA4 or CTLA-4 (cytotoxic T-lymphocyte-associated protein 4), also known as CD152 (cluster of differentiation 152), is a protein receptor that, functioning as an immune checkpoint, downregulates immune responses. CTLA4 is constitutively expressed in Tregs but only upregulated in conventional T cells after activation. It acts as an "off" switch when bound to CD80 or CD86 on the surface of antigen-presenting cells. |
| SKU | GSCRPT-M00570 |
|---|---|
| Supplier Part Number | M00570 |
| UM | EA |
| UNSPSC | 41106514 |
| Manufacturer | GenScript USA Inc |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.00 |
| Lead Time | 7 Business Days |
| Hazardous | N |