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Genscript CHO-K1/human CD47 Stable Cell Line
Genscript CHO-K1/human CD47 Stable Cell Line - GSCRPT (Additional S&H or Hazmat Fees May Apply)
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$8,925.00
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$8,925.00
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NETA PART: | GSCRPT-M00581 |
MFG.PART: | M00581 |
UNSPSC: | 41106514 |
Manufacturer: | GenScript USA Inc |
Overview
Product Description | Recombinant CHO-K1 cells stably overexpress Homo sapiens CD47 molecule (CD47) on the cell surface. The surface expression of CD47 is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screening antibodies against CD47 or to measure binding affinity between CD47 and anti-CD47 antibodies. |
Applications | Cell-based binding assay or use as immunogen |
Product Introduction
Expressed Gene | Codon optimized from NM_001777.3; no expressed tags |
Target Protein | NP_001768.1 |
Host Cell | CHO-K1 |
Size | 2 vials of frozen cells (>1×106 per vial in 1 ml) |
Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
Mycoplasma Status | Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza). |
Stability | Stable through more than 15 passages with no significant changes in assay performance or expression profile. |
Culture Conditions
Culture Properties | Adherent |
Freeze Medium | 95% complete growth medium, 5% DMSO (Cat. No.D2650, Sigma) |
Complete Growth Medium | Hams F-12K (Kaighns) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco) |
Culture Medium | Hams F-12K (Kaighns) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco), 4 μg/ml Puromycin (Cat. No. A11138-03, Gibco) |
Background
Gene Synonyms | IAP, MER6, OA3 |
Background | CD47 (Cluster of Differentiation 47), also known as integrin-associated protein (IAP), is a transmembrane protein that in humans is encoded by CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα). CD47 is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. Furthermore, it plays a key role in immune and angiogenic responses. CD47 is ubiquitously expressed in human cells and has been found to be overexpressed in many different tumor cells. |
SKU | GSCRPT-M00581 |
---|---|
Supplier Part Number | M00581 |
UM | EA |
UNSPSC | 41106514 |
Manufacturer | GenScript USA Inc |
ProductLine | GSCRPT |
Qty | 1 |
MinOrderQty | 1 |
Weight | 7.00 |
Lead Time | 7 Business Days |
Hazardous | N |