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Genscript CHO-K1/human CD32A 131His Stable Cell Line
Genscript CHO-K1/human CD32A 131His Stable Cell Line - GSCRPT (Additional S&H or Hazmat Fees May Apply)
List Price
$9,371.25
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$9,371.25
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| NETA PART: | GSCRPT-M00598 |
| MFG.PART: | M00598 |
| UNSPSC: | 41106514 |
| Manufacturer: | GenScript USA Inc |
Overview
| Product Description | Recombinant CHO-K1 cells stably overexpress human immunoglobulin gamma Fc region receptor II-a (FcγRIIa / CD32A 131His). The surface expression of CD32A 131His is validated by FACS analysis. This stable cell line product is designed for measuring binding affinity and stability of Fc region of antibodies and Fc fusion proteins, or antibody based biologics binding with CD32A 131His. GenScript also offers CD32A 131Arg stable cell line (Cat. No. M00887) for FcγRIIa polymorphism study. |
| Applications | Cell-based binding assay; use as immunogen for antibody development |
Product Introduction
| Expressed Gene | Codon optimized from NM_001136219.1; no expressed tags |
| Target Protein | NP_001129691.1 |
| Host Cell | CHO-K1 |
| Quantity | Two vials of frozen cells (>1×106 per vial in 1 ml) |
| Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status | Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza). |
Culture Conditions
| Culture Properties | Adherent |
| Freeze Medium | 95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma) |
| Complete Growth Medium | Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco) |
| Culture Medium | Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Gibco) |
Background
| Gene Synonyms | CD32; FCG2; FcGR; CD32A; CDw32; FCGR2; IGFR2; FCGR2A1 |
| Background | CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loop acts to lower the production of IgG by B cells when there is a surplus in the body. Monoclonal antibodies can distinguish between CD32A and CD32B. |
| SKU | GSCRPT-M00598 |
|---|---|
| Supplier Part Number | M00598 |
| UM | EA |
| UNSPSC | 41106514 |
| Manufacturer | GenScript USA Inc |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.00 |
| Lead Time | 7 Business Days |
| Hazardous | N |