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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN279 |
| Description | Anti-Beta-mannosidase Antibody, clone 1B3.1 |
| Alternate Names |
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| Background Information | Beta-mannosidase, also known as Lysosomal beta A mannosidase or Mannanase, or Mannase, and encoded by the gene MANBA/MANB1, is an exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of all N-linked glycoprotein oligosaccharides. Beta-mannosidase is localized to the lysosome. Beta-mannosidase is expressed in many cell types including fibroblasts, neutrophils and even neurons and glia. Defects in Beta-mannosidase may be associated with a mild disorder that affects peripheral and central nervous system myelin health and is called Mannosidoisis beta A. It is a lysosomal storage disorder that results in a mixture of clinical features from mental retardation, hearing loss and speech activities. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Beta-mannosidase Antibody, clone 1B3.1 is a highly specific mouse monoclonal antibody, that targets Mannosidase & has been tested in western blotting & IHC (Paraffin). |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Beta-mannosidase in human cerebral cortex tissue. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human Beta-mannosidase. |
| Clone | 1B3.1 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~80 kDa observed. The calculated molecular weight of this protein is 100 kDa. However, this protein undergoes post-translational modification, and may be observed at ~110 kDa, ~100 kDa, ~91 kDa, ~86 kDa, ~84 kDa, and ~75 kDa (Sopher, B. L., et al. (1993). Biochem J. 289(Pt 2):343-347.). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HepG2 cell lysate. Western Blotting Analysis: 0.2 µg/mL of this antibody detected Beta-mannosidase in 10 µg of HepG2 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |