Takara pmRi-ZsGreen1 Vector \20 ug

Takara The pmRi-ZsGreen1 Vector is a Tet-inducible mammalian expression vector that allows the coexpression of a user-generated microRNA (miRNA) and a green fluorescent protein, ZsGreen1, to be controlled by a Tet System transactivator and doxycycline. The TRE-based promoter, P_Tight, regulates the expression of the ZsGreen1 mRNA transcript, which contains your miRNA precursor seq-uence embedded in its 3 UTR. Inducibility requires that a Tet System transactivator (e.g. Tet-On Advanced) also be expressed in the target cells. To select stable cell lines, the pmRi-ZsGreen1 Vector must be cotransfected with one of the provided linear selection markers. - TAKBGN (Additional S&H or Hazmat Fees May Apply)