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Key Specifications Table
| Key Applications | Format |
|---|---|
| APA | Purified |
| Description | |
|---|---|
| Catalogue Number | 14-1121 |
| Description | MultiDsk-Ubiquitin-binding protein reagent |
| Alternate Names |
|
| Background Information | MultiDsk - Ubiquitin-binding protein reagent is a GST fusion protein containing an array of five ubiquitin-associated (UBA) domains from the yeast Ub-binding protein Dsk2 (aa 327-373; UniProt P48510). MultiDsk binds and protects ubiquitinated proteins from deubiquitination. When immobilized on glutathione resins, MultiDsk can be used for affinity pull-down of mono- and poly-ubiquitinated proteins from cell extracts and tissue homogenates. This construct also contains a C-terminal 6xHis sequence and an internal 6xHis sequence between the N-terminal GST and the 5XUBA sequence (PMID 23056298). MultiDsk is expressed in and purified from Rosetta (DE3)pLysS strain of E. coli (Cat. No. 70956). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified ubiquitin-binding protein in buffer containing 25mM Tris-HCl pH7.5, 150mM NaCl, 2mM β-mercaptoethanol with 50% glycerol without preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This MultiDsk-Ubiquitin-binding protein reagent is validated for use in Affinity Precipitation Assays for the pull-down of mono- and poly-ubiquitinated proteins. |
| Key Applications |
|
| Application Notes | Affinity Precipitation Assay Analysis: A representative lot of MultiDsk-Ubiquitin-binding protein was immobilized on agarose beads and used to precipitate ubiquitinated proteins from yeast and HEK293 whole cell extracts. (Wilson, D.M., et al. (2012) PLoS One. 7(10):e46398). Affinity Precipitation Assay Analysis: A representative lot of MultiDsk-Ubiquitin-binding protein was immobilized on agarose beads and used to pull down ubiquitinated proteins from yeast whole cell extracts. (Wilson, D.M., et al. (2013) Cell. 154:983-995). |
| Biological Information | |
|---|---|
| Immunogen | This is an protein reagent, not an antibody as it is a yeast protein, with a GST tag and a His tag, expressed in e. coli. |
| Epitope | Ubiquitin |
| Clone | N/A |
| Concentration | Please refer to lot specific datasheet. |
| Specificity | Mono- and poly-ubiquitinated proteins |
| Source | Yeast protein, with a GST tag and a His tag, expressed in e. coli. |
| Gene Symbol |
|
| Purification Method | Affinity |
| UniProt Number |
|
| Molecular Weight | Multiple bands observed. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Affinity Precipitation in yeast whole cell extract. Affinity Precipitation Assay Analysis: Ubiquitinated proteins were isolated from 1.0 mg yeast whole cell extract by MultiDsk-Ubiquitin-binding protein reagent (7.8 ug to 31.3 ug MultiDsk per pull-down) immobilized on agarose beads |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -80°C from date of receipt. Shipped on Dry Ice. Upon delivery aliquot and store at -80°C. Avoid freeze / thaw cycle |
| Packaging Information | |
|---|---|
| Material Size | 15 µL |