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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB3849-I |
| Replaces | AB3849 |
| Description | Anti-phospho SMAD2 Antibody (Ser465/467) |
| Alternate Names |
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| Background Information | Mothers against decapentaplegic homolog 2 (UniProt Q15796; also known as hMAD-2, hSMAD2, JV18-1, MAD homolog 2, Mad-related protein 2, Mothers against DPP homolog 2, SMAD 2, SMAD family member 2, Smad2) is encoded by the SMAD2 (also known as MADH2, MADR2) gene (Gene ID 4087) in human. Smad2 is a receptor-regulated intracellular signal transducer and transcriptional modulator activated by transforming growth factor TGF-beta and activin type 1 receptor kinases. In response to TGF-beta signal, smad2 is recruited to the activated TGF-beta receptor through its interaction with the SMAD anchor for receptor activation (SARA) protein, where it is phosphorylated by the TGF-beta receptor. The phosphorylation results in its dissociation from SARA and association with another family member smad4. The smad2-smad4 complex then translocates into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. Smad2 can also be phosphorylated by activin type 1 receptor kinase, and thereby transduce activin-dependent signaling events. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-phospho SMAD2 Antibody (Ser465/467) is an antibody against phospho SMAD2 for use in Western Blotting. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Ser465/467 of human Smad2. |
| Epitope | Phospho-Ser465/467. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This antibody detects Smad2 Ser465/467 phosphorylation in TGFbeta-stimulated, but not unstimulated HepG2 cells. The corresponding target phosphoserine residues in the alternatively spliced human Smad2Deltaexon3 (Isoform Short) are pSer435 & pSer437 (UniProt Q15796-2). |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with Mouse, Rat, Bovine, Xenopus, Zebrafish, Chicken based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Modifications |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~53 kDa. 52.31 kDa (Isoform Long) and 48.96 kDa (Isoform Short; Smad2Deltaexon3) calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HepG2 cell lysate. Western Blotting Analysis: 2.0 µg/mL of this antibody detected Smad2 Ser465/467 phosphorylation in 10 µg lysate from TGFbeta-stimulated, but not unstimulated, HepG2 cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |