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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IP | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE1828 |
| Description | Anti-FOXK2 Antibody |
| Alternate Names |
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| Background Information | Forkhead box protein K2 (UniProt Q01167; also known as FOXK2, Cellular transcription factor ILF-1, Interleukin enhancer-binding factor 1) is encoded by the FOXK2 (also known as ILF, ILF-1, ILF1) gene (Gene ID 3607) in human. FOXK2 is a transcription factor involved in cell proliferation and survival. FOXK1 and FOXK2 belong to the family of Fox transcription factors characterized by the forkhead DNA-binding domains. However, FOXK1 and FOXK2 can be distinguished from other Fox transcription factors by the presence of the forkhead-associated (FHA) domains. FOXK2 interacts with BRCA1-associated protein 1 (BAP1) through the FHA domain in a BAP1 Thr493 phosphorylation-dependent manner. BAP1 in turn recruits HCF-1, thereby forming a ternary complex at the FOXK2 target genes sites, where BAP1 represses FOXK2 target genes expression in a BAP1 DUB activity-dependent manner. |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Rabbit polyclonal serum with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-FOXK2 Antibody is validated for use in Western Blotting, Immunoprecipitation for the detection of FOXK2 . |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: A representative lot immunoprecipitated FOXK2 in HeLa nuclear extract (Data courtesy of El Bachir Affar, University of Montreal). Western Blotting Analysis: A representative lot detected FOXK2 in tag-affinity purified BAP1 complex from HeLa cells expressing Flag-HA-tagged wild-type BAP1 or catalytically dead C91S BAP1 mutant (Mashtalir, N., et al. (2014). Mol. Cell. 54(3):392-406). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to the C-terminal region of human FOXK2. |
| Epitope | C-terminal |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | Expected to react with isoforms 1&2, but not isoform 3. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| Molecular Weight | ~78 kDa observed. Isoform 1 (69.1 kDa calculated); Isoform 2 (64.3 kDa calculated). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa nuclear extract. Western Blotting Analysis: A 1:500 dilution of this antibody detected FOXK2 in 10 µg of HeLa nuclear extract. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |