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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IP, ICC, IHC, ChIP | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE1950 |
| Description | Anti-DBC1 Antibody |
| Alternate Names |
|
| Background Information | Cell cycle and apoptosis regulator protein 2 (UniProt Q8N163; also known as Cell division cycle and apoptosis regulator protein 2, DBC-1, DBC.1, DBIRD complex subunit KIAA1967, Deleted in breast cancer 1, p30 DBC, p30 DBC protein) is encoded by the CCAR2 (also known as DBC1, KIAA1967, NET35) gene (Gene ID 57805) in human. Deleted in breast cancer gene 1 protein (DBC1) is a nuclear protein involved in regulating functions of nuclear receptors (NRs), including androgen receptor (AR) and estrogen receptor (ER) alpha and beta. DBC1 stabilizes the interaction between chicken ovalbumin upstream promoter transcription factor I (COUP-TFI) and NCoR by interacting directly with both proteins. DBC1 also interacts with NR complex interacting factor (NRC)-1 to drive the transcription regulation of NRs. Caspase-dependent processing of DBC1 promotes apoptosis, and depletion of DBC1 negatively regulates p53-dependent apoptosis through its specific inhibition of SIRT1. The N-terimus of DBC1 is shown to interact with BRCA1 C-terminal BRCT domain and the expression of DBC1 represses BRCA1-mediated transactivation of the SIRT1 promoter. DBC1 is also a negative regulator against the ligand-dependent transcriptional activation function of ERbeta. The DBC1 protein encoded by the CCAR2 gene is not the same as the protein encoded by the BRINP1 gene, which also goes by the same acronym DBC1 for "Deleted in bladder cancer protein 1". |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing PBS with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect DBC1 using this Anti-DBC1 Antibody validated for use in Western Blotting, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Chromatin Immunoprecipitation (ChIP). |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected DBC1 in HeLa cell lysate (Hiraike, H., et al. (2010). British Journal of Cancer. 102:1061-1067). Western Blotting Analysis: A representative lot detected DBC1 in MDA-MB-231 cell lysate (Koyama, S., et al. (2010). BBRC. 392:357-362). Immunoprecipitation Analysis: A representative lot immunoprecipitated DBC1 in HeLa cell lysate (Hiraike, H., et al. (2010). British Journal of Cancer. 102:1061-1067). Immunocytochemistry Analysis: A representative lot detected DBC1 in MCF7 cells (Hiraike, H., et al. (2010). British Journal of Cancer. 102:1061-1067). Immunocytochemistry Analysis: A representative lot detected DBC1 in MCF7 and T47D cells (Koyama, S., et al. (2010). BBRC. 392:357-362). Immunohistochemistry Analysis: A representative lot detected DBC1 in ductal epithelium and adipose regions of human breast tissue sections (Hiraike, H., et al. (2010). British Journal of Cancer. 102:1061-1067). Immunohistochemistry Analysis: A representative lot detected DBC1 in human breast cancer tissue (Hiraike, H., et al. (2011). Experimental and Therapeutic Medicane. 2:1105-1109). Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected DBC1 occupancy at the SIRT1 gene promoter region (1354–1902) known to be targeted by BRCA1 (Hiraike, H., et al. (2010). Br. J. Cancer. 102(6):1061-1067). |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to human DBC1. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | Expected to reacts with both spliced isoforms. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| Molecular Weight | ~130 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in MCF7 cell lysate. Western Blotting Analysis: 2.0 µg/mL of this antibody detected DBC1 in 10 µg of MCF7 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 200 μg |