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Protein is expressed in E.coli. The protein is manufactured without the use of any animal products/reagents and is considered Animal-Free and can be used in protocols where possible transmisson of animal viruses and contamination must be avoided.
Product endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Description
Catalogue Number
GF319
Description
BMP-2, Human Recombinant Animal Free
Alternate Names
BMP2
BMP 2A
BMP 2
BMP2A
bone morphogenetic protein 2
Background Information
BMP-2 is a potent osteoinductive cytokine, capable of inducing bone and cartilage formation in association with an osteoconductive carrier such as collagen and synthetic hydroxyapatite. In addition to its osteogenic activity, BMP-2 appears to play an important role in cardiac morphogenesis, and is expressed in a variety of other tissues including lung, liver, spleen, prostate, ovary, and small intestine. Recombinant human BMP-2 is a 26.0 kDa homodimeric disulfide-linked protein consisting of two identical 115 amino acid chains.
Product Information
Presentation
Product is presented in 0.1% TFA and is filtered through a 0.2 micron filter before lyophilization.
Quality Level
MQ100
Biological Information
Purity
Product endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Source
Protein is expressed in E.coli. The protein is manufactured without the use of any animal products/reagents and is considered Animal-Free and can be used in protocols where possible transmisson of animal viruses and contamination must be avoided.
UniProt Number
P12643
Product Usage Statements
Quality Assurance
Bioactivity assay: Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 0.5-1.0 μg/ml.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Store at -20°C for up to 4 months from date of receipt Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein and store in working aliquots at -20°C.