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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC287 |
| Description | Anti-PXR Antibody, clone 1F10.1 |
| Alternate Names |
|
| Background Information | Nuclear receptor subfamily 1 group I member 2 (UniProt O75469; also known as Orphan nuclear receptor PAR1, Orphan nuclear receptor PXR, Pregnane X nuclear receptor, Steroid and xenobiotic receptor, SXR) is encoded by the NR1I2 (also known as BXR, PAR, PRR, PXR, SAR, SXR, ONR1) gene (Gene ID 8856) in human. Pregnane X receptor (PXR) is a nuclear receptor that displays similarity to PPAR and RXR. PXR is activated by a large number of endogenous and exogenous chemicals including steroids and antibiotics. One of the key roles of PXR is the induction of CYP3A4, a P450 enzyme involved in drug metabolism. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect PXR using this Anti-PXR Antibody, clone 1F10.1 validated for use in Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 1.0 µg/mL from a representative lot detected PXR in 10 µg of human liver tissue lysate. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human PXR. |
| Clone | 1F10.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Epitope is present in all 7 alternative spliced isoforms (isoform 1A/PRR1-A, 1B/PRR1-B, 1C/PRR1-C, 2A/PRR2-A, 2B/PRR2-V, 2C/PRR2-C, and isoform 3). |
| Isotype | IgG2bκ |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with Chimpanzee, Gorilla based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| Molecular Weight | ~53 kDa observed. Uniprot describes 7 isoforms produced by alternative splicing ranging from ~40 kDa-54 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human colon tissue lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected PXR in 10 µg of human colon tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |