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Genscript cGMP ELISA Detection Kit
Genscript cGMP ELISA Detection Kit - GSCRPT (Additional S&H or Hazmat Fees May Apply)
List Price
$297.00
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$297.00
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| NETA PART: | GSCRPT-L00461 |
| MFG.PART: | L00461 |
| UNSPSC: | 41105322 |
| Manufacturer: | GenScript USA Inc |
Description
Kit DescriptionGenScript cGMP ELISA Detection Kit is a competition enzyme-linked immunoassay which can be used for quantitative detection of cGMP (Guanosine 3,5-cyclic monophosphate) in samples such as serum, plasma, saliva, cell culture supernatant, and urine. cGMP is an important secondary messenger in signal transduction pathways. It is a common regulator of ion channel conductance, glycogenolysis, and cellular apoptosis. cGMP is a cyclic nucleotide derived from guanosine triphosphate (GTP). The cellular production of cGMP is mediated by Guanylate cyclase (GC). It activates the cGMP-dependent protein kinases which in turn phosphorylate several downstream protein targets. cGMP has an effect on the regulation of cAMP levels by activating or inhibiting specific phosphodiesterases (PDEs). The anti-IgG Capture Plate is pre-coated with fixed amount of Goat anti-rabbit IgG to capture Rabbit Anti-cGMP Polyclonal Antibody. When free cGMP or specimen and HRP-cGMP conjugate are added to the well, they compete in the solution to interact with the cGMP antibody captured on the plate. Other unbound molecules are removed by a wash step. The cGMP-HRP reacts with TMB substrate to develop a blue product in the solution. The reaction is stopped by adding stop solution and the color turns yellow which can be read at 450 nm by a Microtiter plate reader. Using the standard curve, the amount of cGMP present in the unknown samples can be calculated by transforming its absorbance value. |
Overview
| Key Features | Key FeaturesApplicationQuantitative detection of cGMP in samples such as serum, plasma, saliva, cell culture supernatant, and urine. StorageThe unopened kit is stable for at least 12 months if stored at 2-8 °C, and the opened kit is stable for up to 2 weeksat 2-8 °C. Do not freeze the kit. Kit Components
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Product Introduction
| Storage | Store at 4°C |
| SKU | GSCRPT-L00461 |
|---|---|
| Supplier Part Number | L00461 |
| UM | KT |
| UNSPSC | 41105322 |
| Manufacturer | GenScript USA Inc |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.00 |
| Lead Time | 7 Business Days |
| Hazardous | Y |