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Genscript CHO-K1/OPRM1/Gα15 Stable Cell Line
Genscript CHO-K1/OPRM1/Gα15 Stable Cell Line - GSCRPT (Additional S&H or Hazmat Fees May Apply)
List Price
$8,925.00
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$8,925.00
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NETA PART: | GSCRPT-M00304 |
MFG.PART: | M00304 |
UNSPSC: | 41105322 |
Manufacturer: | GenScript USA Inc |
Overview
Product Description | Recombinant CHO-K1 cells stably co-overexpress human opioid receptor mu 1 (OPRM1) and G15 alpha subunit. Naturally, OPRM1 is an inhibitory G-protein coupled receptor that activates the Gi alpha subunit, inhibiting adenylate cyclase activity, lowering cAMP levels naturally. But when Gα15 and OPRM1 are co-expressed, the cells can also conduct Gq-coupled signal transduction and mobilize intracellular calcium following certain ligand binding. This cell line is recommended for screening and functional validating agonists of OPRM1. |
Applications | Screening and functional validation of OPRM1 agonists (e.g. Calcium flux assay, cAMP assay) |
Product Introduction
Expressed Gene | Codon Optimized from NM_000914; no expressed tags |
Target Protein | NP_000905 |
Host Cell | CHO-K1/Gα15 |
Quantity | 2 vials of frozen cells (> 1×106 per vial in 1 ml) |
Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
Mycoplasma Status | Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No.LT07-318, Lonza). |
Stability | Stable through more than 16 passages with no significant changes in assay performance or expression profile. |
Culture Conditions
Culture Properties | Adherent |
Freeze Medium | 45% Ham’s F-12K (Kaighns) Medium (Cat. No. 21127-022, Gibco), 45% FBS (Cat. No. 10099-141, Gibco), 10% (V/V) DMSO (Cat. No. D2650, Sigma) |
Complete Growth Medium | Hams F-12K (Kaighns) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco) |
Culture Medium | Ham’s F-12K (Kaighn’s) Meidum (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco), 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. No. 10687010, Invitrogen) |
Background
Gene Synonyms | MOR-1, MOR1, KIAA0403, OPRM |
Background | Opioid receptor mu 1 (oprm1), also known as µ-opioid receptor, is the principal site of action in the brain by which morphine, other opiate drugs of abuse, and endogenous opioid peptides that affect analgesia and alter mood. Opioid receptors belong to the rhodopsin family of G protein-coupled receptors (GPCRs). The three types of opioid receptors (μ, δ, and κ) have been shown to associate with each other in a homotypic or heterotypic fashion when expressed in heterologous cells. A member of the seven-transmembrane domain (TM) G protein-coupled receptor (GPCR) superfamily, the µ-opioid receptor modulates ion channels and second messenger effectors in an opioid agonist-dependent fashion that is reversible by the classic opiate antagonist naloxone. |
SKU | GSCRPT-M00304 |
---|---|
Supplier Part Number | M00304 |
UM | UN |
UNSPSC | 41105322 |
Manufacturer | GenScript USA Inc |
ProductLine | GSCRPT |
Qty | 1 |
MinOrderQty | 1 |
Weight | 7.00 |
Lead Time | 7 Business Days |
Hazardous | N |