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Cell Signaling Sara (D5x4f) Rabbit mAb
Cell Signaling Sara (D5x4f) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
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$327.00
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$327.00
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NETA PART: | CSIG-13285S |
MFG.PART: | 13285S |
UNSPSC: | 41116111 |
Manufacturer: | Cell Signaling |
Size | 100 µl |
Reactivity | H |
Sensitivity | Endogenous |
Molecular Weight (kDa) | 190, 210 |
Source/Isotype | Rabbit IgG |
Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:100} |
Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
Specificity/Sensitivity | Sara (D5X4F) Rabbit mAb recognizes endogenous levels of total Sara protein. This antibody also recognizes a non-specific band of unknown origin at 50 kDa. |
Species Reactivity | Human |
Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val488 of human Sara protein. |
Background | The Smad anchor for receptor activation (SARA, ZFYVE9) protein is an FYVE domain-containing protein originally identified as a regulator of TGF-β signaling (1). FYVE domains are zinc finger-like domains that bind to phosphatidylinositol 3-phosphate and are responsible for endosomal trafficking (2). While the role of Sara in TGF-β signaling has been questioned (3,4), early research studies demonstrate that Sara enhances TGF-β signaling by binding and recruiting non-activated Smad2 and Smad3 to the TGF-β receptor complex (1). Upon Smad2 activation, Sara dissociates from the complex while phosphorylated Smad2/3 translocates to the nucleus to bind to the common Smad, Smad4. Sara can also function as an anchor for the protein phosphatase 1 (PP1c) catalytic subunit, which is involved in the Smad7-mediated dephosphorylation of TGF-β type I receptor (5,6). Additional research studies show that expression of Sara plays a critical role in maintenance of the epithelial cell phenotype and that expression is regulated during the epithelial-to-mesenchymal transition (EMT) and fibrosis (7,8). |
SKU | CSIG-13285S |
---|---|
Supplier Part Number | 13285S |
UM | UNIT |
UNSPSC | 41116111 |
Manufacturer | Cell Signaling |
MSDS URL | https://www.cellsignal.com/contents/technical/safety-data-sheet-(sds)/resources-safety-data-sheets |
Temperature | -20C |
ProductLine | CSIG |
Qty | 1 |
MinOrderQty | 1 |
Weight | 7.00 |
Lead Time | 5 Business Days |
Hazardous | N |