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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | ELISA, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB19292 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-ADAM2 Antibody, clone 9D2.2 |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Liquid at 1 mg/mL in 0.02M phosphate buffer, pH 7.6, 0.25M NaCl, and 0.1% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-ADAM2 Antibody, clone 9D2.2 is validated for use in ELISA, WB for the detection of ADAM2. |
| Key Applications |
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| Applications Not Recommended |
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| Application Notes | Western blot: 1:500 - 1:1,000 dilution for detected against sperm lysates, using standard alkaline phosphatase with NBT/BCIP detection. Chemiluminescent detection may permit higher dilutions. Not effective for immunohistochemistry. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant mouse fertilin produced by baculovirus expressin in SF9 cells. |
| Clone | 9D2.2 |
| Host | Mouse |
| Specificity | Recognizes mouse fertilin beta by immunoblot, staining of bands in a 46-48 kDa range (multiple bands are due to variable protein glycosylation). |
| Isotype | IgG2b |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This member is a subunit of an integral sperm membrane glycoprotein called fertilin, which plays an important role in sperm-egg interactions. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q99965 # Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. Could have a direct role in sperm-zona binding or migration of sperm from the uterus into the oviduct. Interactions with egg membrane could be mediated via binding between its disintegrin-like domain to one or more integrins receptors on the egg. This is a non catalytic metalloprotease-like protein. SIZE: 735 amino acids; 82457 Da SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Expressed specifically in spermatogenic cells in the seminiferous cells. Not detected in fetal tissues. DOMAIN:SwissProt: Q99965 A tripeptide motif (FEE) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding. PTM: The prodomain and the metalloprotease domain are cleaved during the epididymal maturation of the spermatozoa. SIMILARITY: Contains 1 disintegrin domain. & Contains 1 EGF-like domain. & Contains 1 peptidase M12B domain. MISCELLANEOUS: In mammals, exists as a heterodimer composed of an alpha and beta subunits. In human, fertilin subunit alpha is a pseudogene. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |