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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, M, B, Ch, Po, Rb | IP, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-465 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-GRK 2/3 (βARK 1/2) Antibody, clone C5/1.1 |
| Alternate Names |
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| Background Information | The beta-adrenergic receptor kinase specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the ADRBK2 enzyme, also known as GRK3, has 85% amino acid similarity with ADRBK1, with the protein kinase catalytic domain having 95% similarity. The ADRBK2 mRNA is approximately 8 kilobases with a distribution similar to that of ADRBK1. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function. GRK2 phosphorylates the beta2 adrenergic receptor. It is a ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl containing 0.05% sodium azide. Frozen solution. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect GRK 2/3 (βARK 1/2) using this Anti-GRK 2/3 (βARK 1/2) Antibody, clone C5/1.1 validated for use in IP & WB. |
| Key Applications |
|
| Application Notes | Immunoprecipitation: A previous lot of this antibody was used to immunoprecipitate GRK2/3 from transfected cells, as shown by an independent laboratory. |
| Biological Information | |
|---|---|
| Immunogen | Partial GST fusion protein corresponding to the GRK3 (βARK2) carboxyl terminus (a.a. 467-688). This immunogen shares homology with GRK2 (βARK1). The minimal epitope recognized by the antibody is a.a. 483-485. Clone C5/1.1. |
| Epitope | a.a. 483-485 |
| Clone | C5/1.1 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody recognizes GRK2/3, Mr 80 kDa. Additional bands at 85 and 50 kDa are also detected when using higher concentrations of the antibody. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. |
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P25098 # Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. SIZE: 689 amino acids; 79574 Da SUBUNIT: Interacts with GIT1 (By similarity). SIMILARITY: SwissProt: P25098 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain. & Contains 1 RGS domain. ... hide » see more » |
| Molecular Weight | 80 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by immunoblot on RIPA lysates from mouse 3T3/A31 fibroblasts, bovine brain cytosol preparation, rat L6 myoblasts, human A431 carcinoma or human HeLa cell lysates. Western Blot Analysis: 0.1-1 µg/mL of this lot detected GRK2/3 (βARK 1/2) in RIPA lysates from mouse 3T3/A31 fibroblasts. Previous lots of this antibody detected GRK2/3 in bovine brain cytosol preparation, rat L6 myoblasts, human A431 carcinoma and human HeLa cell RIPA lysates. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |