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Key Specifications Table
Species Reactivity | Key Applications | Host | Format | Antibody Type |
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M, R | WB | M | Purified | Monoclonal Antibody |
Description | |
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Catalogue Number | 05-673 |
Brand Family | Upstate |
Trade Name |
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Description | Anti-phospho-Acetyl CoA Carboxylase (Ser79) Antibody, clone BK102 |
Product Information | |
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Format | Purified |
Control |
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Presentation | Protein A Purified immunoglobulin in 0.1M Tris-Glycine, 0.15M NaCl and 0.05% sodium azide, pH 7.4. |
Quality Level | MQ100 |
Applications | |
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Application | Detect phospho-Acetyl CoA Carboxylase (Ser79) using this Anti-phospho-Acetyl CoA Carboxylase (Ser79) Antibody, clone BK102 validated for use in WB. |
Key Applications |
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Biological Information | |
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Immunogen | Synthetic peptide corresponding to rat Acetyl CoA Carboxylase |
Clone | BK102 |
Host | Mouse |
Specificity | Recognizes phosphorylated Acetyl CoA Carboxylase (Ser79). |
Isotype | IgG1λ |
Species Reactivity |
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Antibody Type | Monoclonal Antibody |
Entrez Gene Number |
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Entrez Gene Summary | Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5 sequence and encoding distinct isoforms have been found for this gene. |
Gene Symbol |
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Modifications |
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Purification Method | Protein G Purified |
UniProt Number |
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UniProt Summary | FUNCTION: SwissProt: Q13085 # Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase. COFACTOR: Biotin. & Binds 2 manganese ions per subunit. SIZE: 2346 amino acids; 265554 Da SUBUNIT: Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver. PTM: Phosphorylation on Ser-1263 is required for interaction with BRCA1. SIMILARITY: SwissProt: Q13085 ## Contains 1 ATP-grasp domain. & Contains 1 biotin carboxylation domain. & Contains 1 biotinyl-binding domain. & Contains 1 carboxyltransferase domain. |
Molecular Weight | 257 kDa |
Product Usage Statements | |
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Quality Assurance | Routinely evaluated by immunoblot on HeLa acid extracts |
Usage Statement |
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Storage and Shipping Information | |
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Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
Packaging Information | |
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Material Size | 200 µg |