The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
Millipore Detect 53bp1 With Anti-53bp1 Antibody, Clone Bp13 (Mouse Monoclonal Antibody), That Has Been Shown To Work In Ip, Wb, Icc. - Mill (Additional S&H or Hazmat Fees May Apply)
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Quality Level
MQ100
Applications
Application
Detect 53BP1 with Anti-53BP1 Antibody, clone BP13 (Mouse Monoclonal Antibody), that has been shown to work in IP, WB, ICC.
Key Applications
Immunoprecipitation
Western Blotting
Immunocytochemistry
Application Notes
Does not cross-react with mouse.
Biological Information
Immunogen
Mix of three GST fusion proteins corresponding to residues 1-337, 338-671, and 1331-1664, respectively, of human 53BP1
Clone
clone BP13
Host
Mouse
Specificity
53BP1
Isotype
IgG1
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NM_005657.1
Gene Symbol
TP53BP1
53BP1
p53BP1
p202
FLJ41424
MGC138366
Purification Method
Protein G Purified
UniProt Number
Q12888
UniProt Summary
FUNCTION: SwissProt: Q12888 # May have a role in checkpoint signaling during mitosis (By similarity). Enhances TP53-mediated transcriptional activation. Binds to sites of DNA damage. Plays a role in the response to DNA damage. SIZE: 1972 amino acids; 213574 Da SUBUNIT: Interacts with IFI202A (By similarity). Binds to the central domain of TP53/p53. May form homo-oligomers. Interacts with DCLRE1C. Interacts with histone H2AFX and this requires phosphorylation of H2AFX on Ser-139. Interacts with histone H3 that has been methylated on Lys-79. Does not bind unmethylated histone H3. SUBCELLULAR LOCATION: Nucleus. Kinetochore. Note=Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. PTM: Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding. & Phosphorylated in an ATM-dependent manner in response to DNA damage. SIMILARITY: SwissProt: Q12888 ## Contains 2 BRCT domains.
Molecular Weight
~250kDa
Product Usage Statements
Quality Assurance
routinely evaluated by immunoblot on lysates from HeLa cells
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
