Special Offers
100% Performance Guaranteed
Key Specifications Table
Species Reactivity | Key Applications | Host | Format | Antibody Type |
---|---|---|---|---|
H, M, R | ICC, WB | M | Purified | Monoclonal Antibody |
Description | |
---|---|
Catalogue Number | 05-778 |
Brand Family | Upstate |
Trade Name |
|
Description | Anti-Rho (-A Antibody, -B, -C), clone 55 |
Alternate Names |
|
Background Information | Rho proteins belong to the Ras super family of low molecular weight GTPases, which includes RAS, RHO, RAB, RAN, RAD and ARF. These small GTP binding proteins function as molecular switches that regulate a multitude of biological processes including cell proliferation, apoptosis, differentiation, cytoskeletal reorganization, and membrane trafficking. |
Product Information | |
---|---|
Format | Purified |
Control |
|
Presentation | Purified mouse monoclonal IgG1 in buffer containing BSA containing 0.09% sodium azide and 50% glycerol. |
Quality Level | MQ100 |
Applications | |
---|---|
Application | Detect Rho (-A, -B, -C) using this Anti-Rho (-A Antibody, -B, -C), clone 55 validated for use in IC & WB. |
Key Applications |
|
Application Notes | Immunocytochemistry: This antibody has been reported by an independent laboratory to detect Rho in cells via immunofluorescence. |
Biological Information | |
---|---|
Immunogen | Recombinant human RhoA containing amino acids 1-155. Clone 55. |
Clone | 55 |
Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
Host | Mouse |
Specificity | Recognizes Rho (-A, -B, -C), Mr 22 kDa. |
Isotype | IgG1 |
Species Reactivity |
|
Antibody Type | Monoclonal Antibody |
Entrez Gene Number |
|
Entrez Gene Summary | This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. |
Gene Symbol |
|
Purification Method | Protein G Purified |
UniProt Number |
|
UniProt Summary | FUNCTION: SwissProt: P62745 # Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. SIZE: 196 amino acids; 22123 Da SUBUNIT: Binds ROCK1 and ROCK2. Also binds PKN1/PRK1. Interacts with ARGGEF3, RTKN and AKAP13. SUBCELLULAR LOCATION: Late endosome membrane; Lipid-anchor. Cell membrane; Lipid-anchor. Nucleus. Note=Late endosomal membrane (geranylgeranylated form). Plasma membrane (farnesylated form). Also detected at the nuclear margin and in the nucleus. PTM: Prenylation specifies the subcellular location of RHOB. The farnesylated form is localized to the plasma membrane while the geranylgeranylated form is localized to the endosome. SIMILARITY: SwissProt: P62745 ## Belongs to the small GTPase superfamily. Rho family. MISCELLANEOUS: RHOB is one of the targets of farnesyltransferase inhibitors which are currently under investigation as cancer therapeutics. These elevate the levels of geranylgeranylated RHOB and cause mislocalization, leading to apoptosis and antineoplastic effects. |
Molecular Weight | 22 kDa |
Product Usage Statements | |
---|---|
Quality Assurance | Routinely evaluated by western blot on RIPA lysates from Jurkat or HL-60 cells. Western Blot Analysis: 0.5-2 µg/mL of this lot detected Rho in RIPA lysates from HL-60 cells. A previous lot detected Rho in RIPA lysates from Jurkat cells. |
Usage Statement |
|
Storage and Shipping Information | |
---|---|
Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20ºC may cause glycerol-containing solutions to become frozen during storage. |
Packaging Information | |
---|---|
Material Size | 200 µg |