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Key Specifications Table
Species Reactivity | Key Applications | Host | Format | Antibody Type |
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H | WB | Rb | Affinity Purified | Polyclonal Antibody |
Description | |
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Catalogue Number | 07-488 |
Brand Family | Upstate |
Trade Name |
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Description | Anti-PKD2 Antibody |
Alternate Names |
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Product Information | |
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Format | Affinity Purified |
Control |
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Presentation | Immunoaffinity purified rabbit IgG in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl and 0.05% sodium azide as a preservative. |
Quality Level | MQ100 |
Applications | |
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Application | Anti-PKD2 Antibody detects level of PKD2 & has been published & validated for use in WB. |
Key Applications |
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Application Notes | Immunoblot Analysis: A 1:500-1:1,000 dilution of this antibody detected PKD2 in RIPA lysates from A431 cells that had been stimulated with PMA. |
Biological Information | |
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Immunogen | KLH-conjugated, synthetic peptide corresponding to amino acids 850-865 (GLPTDRDLGGACPPQD) of human protein kinase D2 (PKD2). |
Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
Host | Rabbit |
Specificity | Recognizes PKD2. |
Isotype | IgG |
Species Reactivity |
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Antibody Type | Polyclonal Antibody |
Entrez Gene Number |
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Entrez Gene Summary | The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. |
Gene Symbol |
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Purification Method | ImmunoAffinity Purified |
UniProt Number |
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UniProt Summary | FUNCTION: SwissProt: Q9BZL6 # Calcium-independent, phospholipid-dependent, serine- and threonine-specific protein kinase. SIZE: 878 amino acids; 96722 Da TISSUE SPECIFICITY: Widely expressed. PTM: Autophosphorylated. Phorbol esters stimulates autophosphorylation. Phosphorylation of Ser-876 correlates with the activation status of the kinase. SIMILARITY: SwissProt: Q9BZL6 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 PH domain. & Contains 2 phorbol-ester/DAG-type zinc fingers. & Contains 1 protein kinase domain. |
Molecular Weight | 105 kDa |
Product Usage Statements | |
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Quality Assurance | Routinely evaluated by immunoblot in RIPA lysates from HEK293m cells that had been stimulated with 50 ng/mL of PDBu for 10 minutes or PMA stimulated A431 cells. |
Usage Statement |
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Storage and Shipping Information | |
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Storage Conditions | Maintain for 1 year at 2 to 8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
Packaging Information | |
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Material Size | 200 µL |