| Description | |
|---|---|
| Catalogue Number | 14-343 |
| Brand Family | Upstate |
| Trade Name |
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| Description | FKHR Protein, 100 µg |
| Overview | N-terminal, Glutathione-Stransferase (GST) tagged, full-length fusion protein corresponding to human FKHR |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | N-terminal, Glutathione-Stransferase (GST) tagged, full-length fusion protein corresponding to human FKHR. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | Recombinant human expressed in E. coli |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. |
| Gene Symbol |
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| Purification Method | Glutathione-agarose |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q12778 # Transcription factor. SIZE: 655 amino acids; 69662 Da SUBUNIT: Interacts with LRPPRC (By similarity). SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Shuttles between cytoplasm and nucleus. TISSUE SPECIFICITY: Ubiquitous. PTM: Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Thr-24, Ser-256 and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, which leads to nuclear exclusion and loss of function. Phosphorylation of Ser- 329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR. DISEASE: SwissProt: Q12778 # Chromosomal aberrations involving FOXO1 are a cause of rhabdomyosarcoma 2 (RMS2) [MIM:268220]; also known as alveolar rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator. SIMILARITY: SwissProt: Q12778 ## Contains 1 fork-head DNA-binding domain. |
| Molecular Weight | 105kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by phosphorylation by Akt1 |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | 1 year at -70°C |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |