Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, ICC, IP | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE257 |
| Replaces | MABE515 |
| Description | Anti-RAD51 (rabbit polyclonal) |
| Alternate Names |
|
| Background Information | DNA repair protein RAD51 homolog 1 (UniProt: Q06609; also known as HsRAD51, hRAD51, RAD51 homolog A) is encoded by the RAD51 (also known as RAD51A, RECA) gene (Gene ID: 5888) in human. RAD51 plays an important role in DNA repair through homologous recombination. It is highly expressed in testis and thymus and is also found in small intestine, placenta, colon, pancreas and ovary. It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. It is shown to catalyze the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. It colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. Phosphorylation of RAD51 at threonine 309 by checkpoint kinase 1 (CHEK1) enhances its association with chromatin at sites of DNA damage and promotes DNA repair. It binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. RAD51 also plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress and its stress-induced increases are observed in the mitochondria. Mutations in RAD51 gene are observed in cases of invasive ductal carcinoma and in Fanconi anemia. (Ref.: Hilario, J., et al. (2009). Proc. Natl. Acad. Sci. USA. 106(2);361-368). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-RAD51 Antibody is a Rabbit Polyclonal Antibody for detection of RAD51 also known as DNA repair protein RAD51 homolog 1, HsRAD51 & has been validated in WB, ICC & IP. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: 1:500 from a representative lot detected RAD51 in HeLa and A431 cells. Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated RAD51 in HeLa cell lysate. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Biological Information | |
|---|---|
| Immunogen | His-tagged full-length human recombinant RAD51. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects DNA repair protein RAD51. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse, Rat. Predicted to react with Bovine, Canine, Horse based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: May participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. SUBUNIT STRUCTURE: Interacts with BRCA1, BRCA2 and either directly or indirectly with p53. Interacts with XRCC3, RAD54L and RAD54B. Part of a complex with RAD51C and RAD51B. Interacts with RAD51AP1 and RAD51AP2. Interacts with CHEK1/CHK1, and this may require prior phosphorylation of CHEK1. Interacts with the MND1-PSMC3IP heterodimer (By similarity). Interacts with OBFC2B. Interacts with RPA1. Interacts with PALB2. Interacts with SWI5/C9orf119, and at lower level with SFR1/MEIR5. SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Cytoplasm › perinuclear region. Mitochondrion matrix. Note: Colocalizes with RAD51AP1 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. TISSUE SPECIFICITY: Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast. INDUCTION: Stress-induced increase in the mitochondrial levels is seen. PTM: Phosphorylated. Phosphorylation of Thr-309 by CHEK1/CHK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. INVOLVEMENT IN DISEASE: Defects in RAD51 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case. Ref.28 SEQUENCE SIMILARITIES: Belongs to the RecA family. RAD51 subfamily. Contains 1 HhH domain. |
| Molecular Weight | ~37 kDa observed; 36.97 kDa calculated.. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: A 1:4,000 dilution of this antibody detected RAD51 in HeLa cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |